Research Project:  

  1. Molecular cloning of DPKAP

      Method : cDNA library screening, Northern and Western blot,

                     FPLC,  protein purification, LC/MS/MS, recombinant

                     protein expression and antibody production.

  2. DNA-PK-associated proteins identification.

      Method : Antibody production and affinity chromatagraphy.

  3. Physical and functional interaction of DPKAP and GR.

      Method : CAT assay and Immunoprecipitation.

  4. Limbal stem cells study : Identification of the stem cells marker.

      Method : Differential Display Screening & DNA Microarray

                     Analysis for Identification of the Limbal Stem 

                     Cells Marker.

  5. Biosensor and One Step Quick Diagnosis at  Tyson Bioresearch

      Inc. :  Glucose Meter Project.

◆發表著作

1. Miau LH, Chang CJ, Shen BJ, Tsai WH, Lee SC. 1998. Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBP beta-mediated gene activation. J Biol Chem. 273:10784-10791

2. Shen BJ, Chang CJ, Lee HS, Tsai WH, Miau LH, Lee SC. 1997. Transcriptional induction of the agp/ebp (c/ebp beta) gene by hepatocyte growth factor. DNA Cell Biol. 16:703-711.

3. Miau LH, Chang CJ, Tsai WH, Lee SC. 1997. Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a transcription factor. Mol Cell Biol. 17:230-239.

4. Miau LH, Jan YW, Shen BJ, Tsai WH, Lee HS, Lee SC. 1996. Identification of a novel variant hepatocyte growth factor secreted by spleen-derived stromal cells. Biochem Biophys Res Commun. 223:487-491.

5. Yang TH, Tsai WH*, Lee YM, Lei HY, Lai MY, Chen DS, Yeh NH, Lee SC. 1994. Purification and characterization of nucleolin and its identification as a transcription repressor. Mol Cell Biol. 14:6068-6074.(*equal contribution)

6. Lee YM, Tsai WH, Lai MY, Chen DS, Lee SC. 1993. Induction of liver alpha-1 acid glycoprotein gene expression involves both positive and negative transcription factors. Mol Cell Biol. 13:432-442.

7. Biochemical and Molecular Biological Characterization of Nucleolin and a Novel PHD-finger Protein. (Thesis) 1999