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| HOME | ||||||||||||||||
| Methods | ||||||||||||||||
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| The determination of the molecules involved in the STP encompasses several approaches. The pathway can be visualized by applying the knowledge of interacting substances. The interactions are confirmed by DNA to protein interactions (in vivo in the Y2H) and purified protein interactions (in vitro.) |
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| Yeast Two Hybrid System | ||||||||||||||||
| Prep of Competant Cells / Y2H as applied on Xb10 | ||||||||||||||||
| Isolation of Plasmid DNA from Yeast | ||||||||||||||||
| Ronald lab view | ||||||||||||||||
| Purification and Sequencing of Xb10 interacting library cDNA | ||||||||||||||||
| Y2H Back Screen | ||||||||||||||||
| Xb10 Protein Purificatiuon | ||||||||||||||||
| Northern Blot Analysis of Xb10, WRKY1, WRKY2, and WRKY3 | ||||||||||||||||
| PCR Primer Amplification of WRKY1 | ||||||||||||||||