The DNA in all test subjects was stored in the plasmids pPC86, pPC97, 7653 bp, and 6491bp.
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| The Yeast Two Hybrid System Directly Screening Xb10 and Xb12 with Purified DNA Segments | ||||||||||
| The process of this modification of the yeast two hybrid system is the same as the library screening except for a few alterations: The prey were purified DNA such as Xb10, Xb12, Xb21, Xb3, and other substances that can be directly tested with the bait (either Xb10 or Xb12.) DNA segments are used and mixed with the bait instead of using the rice library genes. The DNA in all test subjects was stored in the plasmids pPC86, pPC97, 7653 bp, and 6491bp. |
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| plasmid DNA | ||||||||||