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Genetic Engineering:
Recombinant DNA

Restriction Enzymes Can Cut DNA into Precise Fragments

  • Restriction enzymes cut only at specific sequences of 4 to 8 bases
  • A long restriction site is rare. Cutting rare sites will produce a few large pieces of DNA.
    • Restriction enzymes with long sites -> small number of large fragments
    • Restriction enzymes with short sites -> large number of small fragments

       
       Number of
      bases
       Probability  

       Average Number
      of Fragments for
      1 Million Bases

       Average Size
      of Fragment
      (Base Pairs)
       

       4

       

       1/256

       

       3907

       

       256

       

       5

       

       1/1024

       

       978

       

       1024

       

       6

       

       1/4096

       

       245

       

       4096

       

       7

       

       1/16384

       

       62

       

       16384

       

       8

       

       1/65536

       

       16

       

       65536

 

 

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