PUBLICATIONS

 

 

Can machines recognize stress in plants?

 

Link: Environmental Chemistry Letters (2003) 1(3): 201–205

Springer Berlin / Heidelberg

ISSN: 1610-3653 (Paper) 1610-3661 (Online)

DOI: 10.1007/s10311-003-0034-7

For a PDF copy of this paper click here

 

Ronald Maldonado Rodríguez¹, Stancho Vaelkanov Pavlov², Alberto Gonzalez Moreno³, Abdullah Okarum¹, Reto Strasser¹

 

(1)  Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

(2)  Department of Mathematics, University Asen Zlatarov, 8010 Burgas, Bulgaria

(3)  INIA Carretera de la Coruña, 28040 Madrid, Spain

 

Keywords:  Artificial neural networks - Chlorophyll a fluorescence - Drought stress - JIP-test - O-J-I-P fluorescence rise - Pea - Pisum sativum - Plants - Self-organizing map – SOM - Kohonen

 

Abstract 

In this paper we show that chlorophyll a fluorescence signals analysed with the self-organizing map (SOM) can be used as a routine tool for the monitoring and classification of pea varieties (Pisum sativum) according to their degree of resistance against drought stress. Fluorescence kinetics measurements were obtained from non-stressed plants. The aim of this study is to evaluate the applicability of artificial intelligence techniques in eco-physiological research. Our goal is to provide a fast tool that will contribute to the knowledge needed to develop strategies that would help to decrease the impact of environmental stress in agriculture and forestry.

 

 

A Methodological Approach for Pattern Recognition System using discriminate analysis and artificial neural networks

 

Link: Proceedings of the 6^th WSEAS Neural Networks, Fuzzy Systems, Evolutionary Computing Conference held in Lisbon, Portugal, 14-18 July 2005.  [496-183]

WSEAS Transactions Journal (Accepted for Publication July 2005)

 

Anna Pérez-Méndez, Elizabeth Torres-Rivas,  Francklin Rivas-Echeverría, Ronald Maldonado-Rodríguez

 

This work is the result of a scientific collaboration between the Escuela de Estadística and the Laboratorio de Sistemas Inteligentes de la Universidad de Los Andes, Mérida, Venezuela and Ronald Maldonado Rodriguez at  the Bioenergetics Laboratory of the  University of Geneva, Switzerland. e-mail: [email protected], [email protected], [email protected], [email protected]

 

Keywords: - Classification, Pattern recognition, Discriminate analysis, Artificial Neural Networks

 

Abstract

In this work it is presented a methodology for the development of a pattern recognition system using classification methods as discriminate analysis and artificial neural networks. In this methodology, the information statistical analysis is contemplated, with the purpose of retaining the observations and the important characteristics that can produce an appropriate classification, and allows, as well, to detect outliers’ observations, and multicolinearity between variables, among other things.

 

 

Pisum sativum classification based on a methodological approach for pattern recognition using discriminant analysis and neural networks

 

Link: Proceedings of the 6^th WSEAS Neural Networks, Fuzzy Systems, Evolutionary Computing held in Lisbon, Portugal 14-18 July 2005 [496-184]

WSEAS Transactions Journal (accepted for publication 07.05)

 

Anna Pérez-Méndez, Ronald Maldonado-Rodríguez, Elizabeth Torres-Rivas, Franklin Rivas-Echeverría.

 

This work is the result of a scientific collaboration between the Escuela de Estadística and the Laboratorio de Sistemas Inteligentes de la Universidad de Los Andes, Mérida, Venezuela, and Ronald Maldonado Rodriguez at the Bioenergetics Laboratory of the University of Geneva, Switzerland. e-mail: [email protected], [email protected], [email protected], [email protected]

 

Keywords: - Classification, Pattern recognition, Discriminate analysis, Artificial Neural Networks

 

@ This paper obtained the Best Student Paper Award for Fuzzy Systems at the 6th WSEAS Lisbon 2005 Conference. Read more here.

 

Abstract

In this work a statistical analysis-based methodological approach for a pattern recognition system using discriminate analysis and neural networks is used for the classification of Pisum sativum (pea) according to the drought resistance. The statistical techniques used in the exploratory analysis are a fundamental tool in the creation of variables sets and observations for the model adjustment in the neural models and in the discriminate models.

 

WSEAS is The World Scientific and Engineering Academy and Society.

 

 

Chlorophyll a fluorescence patterns of six deciduous forest tree species exposed to normal and elevated CO₂

 

R. Maldonado Rodríguez, R. Strasser. Submitted

 

Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

 

Abstract

Artificial Neural Networks (ANN) are able to discover “patterns” in multivariate data. We show that ANN may offer a realistic opportunity to automation of physiological patterns identification and plant stress quantification by using Chlorophyll a Fluorescence (CF) signals as network information input.  Fluorescence is a widely spread technique used in many photosynthesis and eco-physiology research laboratories, and it finds numerous applications, from agriculture and forestry, through marine biology and chronobiology to exobiology research. CF signals are a direct measure of photosynthetic performance in plants and algae. Fluorescence signals are a definitive proof for photosynthesis. Measuring fluorescence is cheap, fast, and non-destructive. In a relative short time, thousands of fluorescence curves can be collected. The task of identification of CF patterns that correlate with other physiological parameters becomes a necessity. The physiological responses of photosynthetic organisms to well-defined stimuli have been observed to be similar, presenting well-defined patterns and indeed this characteristic suggest the possibility to group plants, green algae and cyanobacteria into categories or classes according to their specific fluorescence pattern. Since pattern recognition is the primary emphasis, an ANN seems to be the most logical method of solving this problem. Formed by simulated neurons connected together much the same way the brain's neurons are, ANN are able to associate and generalize without rules. They have solved problems in pattern recognition, robotics, speech processing, financial predicting, and signal processing, to name a few. The Self-Organizing Feature Map (SOM) is a popular ANN. We build a SOM using plant fluorescence signals as stimulatory input and the resulting Fluorotopic Map has shown to be a valuable tool for identification of plant classes. Such classes may vary according to their taxonomy, functional groups, wild type or genetically modified plants, degree of stress effects, etc. We demonstrate a novel methodology for plant stress survey using short video sequences of whole leaf fluorescence analyzed with a Batch-SOM. The new developed technique includes spatio-temporal analysis of fluorescence kinetics obtained from video processing combined with fast fluorescence induction measurements. Using this technique, a new method for recognizing and quantifying plant stress has been developed. We have tested the applicability of this new technique within the Swiss Canopy Crane Project framework (Hofstetten, Basel), where the goal was to compare the photosynthetic performance of forest trees growing in an enriched CO2 atmosphere to that of trees growing in an ambient CO2 atmosphere. Further modelling of leaf photosynthetic performance and up-scaling of the proposed model from the leaf to the forest level, as well as applications in Plant Health Status Survey, Forest Management, Precision Agriculture and Urban Tree Vitality Assessment are discussed. 

 

 

Self-Organizing Map (SOM) for monitoring the evolution of Rhizobium nodulation status in Vigna unguiculata

 

Ronald Maldonado Rodríguez, Patrick Schmitz, Reto Strasser. Submitted

 

Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

 

Abstract        

Chlorophyll a fluorescence is a useful and non-invasive tool to screen for the effects of many biotic and abiotic parameters on photosynthesis in plants. The Chl a fluorescence emitted by leaves after excitation with red light was measured with a portable fluorometer. The collected data showing the polyphasic OJIP Chl a fluorescence rise were analysed using the JIP-test (Strasser and al. 2000) which provides biophysical parameters indicating Photosystem II properties. Seeds of Vigna unguiculata were sterilized before germination. Seedlings were planted in Magenta jars (used for hydroponic cultures) filled with a nitrate-deficient solution (B&D solution). The roots of some of the plants were inoculated with Rhizobium sp. strain NGR234 (10⁹ bacteria/200 µl) four days after germination. The other plants were grown on various concentrations of KNO₃ (0, 0.5, 1, 5, 10 and 20 mM). The plants were followed for 5 weeks. During this time fluorescence measurements were done on the first and second mature leaves. We have use an Artificial Neural Network (Kohonen's Self-Organizing Map or SOM) to analyze the raw fluorescence data. The generated map shows very well defined groups of different concentrations creating a gradient from low to high nitrate content. The Rhizobium inoculated plants in the fluorescence SOM map is moving in time according to the hypothetical nitrogen supply model. This permits us to establish a method for screening the nodulation evolution as well as nitrogen deficiency in vivo on the level of the leaves.

 

 

Ecophysiological responses to summer drought in Pinus halepensis Mill. seedlings of five provenances

 

Rafael Mª Navarro1, David Ariza1, Ronald Maldonado Rodríguez, Francisco Canovas.

In preparation

 

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10 Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland

 

Resumen

En este ensayo se estudia la utilidad de las medidas de la cinética de inducción de fluorescencia de la clorofila de hojas in situ para detectar la respuesta temprana a estrés hídrico moderado de cinco procedencias Pinus halepensis Mill.. Las plantas se sometieron a un ciclo de sequías de 28 días en una cámara de cultivo a 21ºC. Se han encontrado diferencias significativas de supervivencia entre las procedencias a los 21 días, pero no al final del ensayo. La variación en la fluorescencia ha mostrado que la eficiencia potencial del fotosistema II de las plantas sometidas a un estrés hídrico moderado es menor conforme aumenta el nivel de estrés (medido en potencial hídrico) y que esta diferencia varía entre procedencias, por lo que puede representar una medida indirecta del nivel de estrés en etapas previas a la aparición de perdidas generales de supervivencia.

 

 

 

Quality assessment of urban trees: A comparative study of physiological characterisation, airborne imaging and on site fluorescence monitoring by the JIP-test

 

Link: Journal of Plant Physiology.

 160 (1): 81–90 (2003)

 

Urban & Fischer Verlag Publishers

DOI: 10.1078/0176-1617-00917

  For a PDF copy of this article click here

Christian Hermans^{1, 2, a}, Mathias Smeyers³, Ronald Maldonado Rodriguez¹, Murielle Eyletters², Reto J. Strasser¹, Jean-Paul Delhaye² *

1 Bioenergetics Laboratory, University of Geneva, chemin des Embrouchis, CH-1254, Jussy-Geneva, Switzerland

2 Laboratoire d’Agrotechnologies Végétales – CP169, Université Libre de Bruxelles, 50 Av. F.D. Roosevelt, B-1050 Brussels, Belgium

3 Laboratoire de Physiologie Végétale – CP206-2, Université Libre de Bruxelles, Campus Plaine, Bd. du Triomphe, B-1050 Brussels, Belgium

Keywords: Chlorophyll fluorescence – Tree vitality – Urban tree stress factors – JIP-Test – Performance Index

Abstract

The purpose of this paper is to demonstrate the complementary utility of chlorophyll fast fluorescence OJIP transient (from 50µs to 1s) measurements in the aerial study of rows of trees. We identify limitations in photochemical events induced by urban injuries on Platanus acerfolia L., using the JIP-test procedure. The Performance Index (PI_ABS) showed the largest dynamic range to characterise the vitality of trees. Individual trees were graded into three quality groups based on the individual PI_ABS value compared to the overall average for trees in the alley. These groups are: high performers, with PI_ABS 50% higher than the alley average; normal trees, with a deviation from the alley average between –50% and +50%; and poor performers, whose deviation from the alley average was –50% or less. Trees also were grouped into five vitality categories on the basis of a stereoscopic and morphologic observation of the symmetry of tree crowns, percentage of defoliation and reflectance property in the visible and infra-red range. Here, we report a remarkable correlation between the airborne remote sensing data and the on-site fluorescence measurements.

Prompt and delayed Chlorophyll a fluorescence analysis of intact pea leaves treated with photosynthetic herbicides

Petar H. Lambrev, Vassilij N. Goltsev, Ronald Maldonado Rodríguez, Reto J. Strasser

^{1 Faculty of Biophysics. University of Sofia “Kliment Ohridskii”; Sofia, Bulgaria}

^{2 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

Abstract.

A method for testing the activity of photosynthetic herbicides in intact plant objects based on measurements of prompt and millisecond delayed chlorophyll fluorescence was proposed. The activities of Diuron, Atrazine and Dinoseb were determined in pea leaves. Different methods for treatment of leaves were compared in respect to their efficiency.  It was found that manually infiltrating the leaves by vacuum is not suitable for measuring the herbicide activity because the variability is rather high. The most efficient and reliable way of treatment with Urea/Triazine herbicides was by allowing the plants to absorb and transport the substance through the stems. The results demonstrate that quantitative measurements of herbicide activity are possible in intact leaves by means of luminescence methods. Several factors were shown to have significant impact on the final effect such as the need of light for herbicide penetration or the statistical heterogeneity of the sample. Submitted

Effects of Lindane on the Photosynthetic Apparatus of the Cyanobacterium Anabaena: Fluorescence Induction Studies  

and Immunolocalization of Ferredoxin-NADP+ Reductase.

Environmental Sciences & Pollution Research 11 (2) 98–106 (2004)

DOI: http://dx.doi.org/10.1065/espr2003.10.175

 For a PDF copy of this article click here

 

Marta Bueno¹, Maria F. Fillat¹, Reto J. Strasser², Ronald Maldonado-Rodriguez², Nerea Marina¹, Henry Smienk¹, Carlos Gómez-Moreno¹ and Francisco Barja³*

 

1 Department of Biochemistry and Molecular and Cell Biology, Faculty of Sciences, Pedro Cerbuna 12, University of Zaragoza, Spain

2 Bioenergetics Laboratory, University of Geneva, Chemin des Embrouchis 10, CH-1254 Jussy-Geneva, Switzerland

3 Bioenergetics and Microbiology Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

 

Abstract

Intention, Goal, Scope, Background

Cyanobacteria have the natural ability to degrade moderate amounts of organic pollutants. However, when pollutant concentration exceeds the level of tolerance, bleaching of the cells and death occur within 24 hours. Under stress conditions, cyanobacterial response includes the short-term adaptation of the photosynthetic apparatus to light quality, named state transitions. Moreover, prolonged stresses produce changes in the functional organization of phycobilisomes and in the core-complexes of both photosystems, which can result in large changes in the PS II fluorescence yield. The localization of ferredoxin-NADP+ reductase (FNR) at the ends of some peripheral rods of the cyanobacterial phycobilisomes, makes this protein a useful marker to check phycobilisome integrity.

Objective

The goal of this work is to improve the knowledge of the mechanism of action of a very potent pesticide, lindane (γ-hexachlorocyclohexane), in the cyanobacterium Anabaena sp., which can be considered a potential candidate for bioremediation of pesticides. We have studied the effect of lindane on the photosynthetic apparatus of Anabaena using fluorescence induction studies. As ferredoxin-NADP+ reductase plays a key role in the response to oxidative stress in several systems, changes in synthesis, degradation and activity of FNR were analyzed. Immunolocalization of this enzyme was used as a marker of phycobilisome integrity. The knowledge of the changes caused by lindane in the photosynthetic apparatus is essential for rational further design of genetically-modified cyanobacteria with improved biorremediation abilities.

Methods

Polyphasic chlorophyll a fluorescence rise measurements (OJIP) have been used to evaluate the vitality and stress adaptation of the nitrogen-fixing cyanobacterium Anabaena PCC 7119 in the presence of increasing concentrations of lindane. Effects of the pesticide on the ultrastructure have been investigated by electron microscopy, and FNR has been used as a marker of phycobilisome integrity.

Results and Discussion

Cultures of Anabaena sp. treated with moderate amounts of lindane showed a decrease in growth rate followed by a recovery after 72 hours of pesticide treatment. Concentrations of lindane below 5 ppm increased the photosynthetic performance and activity of the cells. Higher amounts of pesticide caused a decrease in these activities which seems to be due to a non-competitive inhibition of PS II. Active PS II units are converted into non-QA reducing, so called heat sink centers. Specific activity and amount of FNR in lindane-treated cells were similar to the values measured in control cultures. Release of FNR from the thylakoid after 48 hours of exposure to 5 ppm of lindane towards the cytoplasm was detected by immunogold labeling and electron microscopy. Conclusions. From these results, we conclude that the photosynthetic performance and activity of the cells are slightly increased in the presence of lindane up to 5 ppm. Moreover, in those conditions, lindane did not produce significant changes in the synthesis, degradation or activity of FNR. The high capability of Anabaena to tolerate lindane makes this cyanobacterium a good candidate for phytoremediation of polluted areas.

Recommendation and Outlook

The results of this study show that cultures of Anabaena PCC 7119 tolerate lindane up to 5 ppm, without significant changes in the photosynthetic vitality index of the cells. However, a slight increase in phycobiliprotein synthesis is observed, which is related to total protein content. This change might be due to degradation of proteins less stable than phycobiliproteins. An identification of the proteins with altered expression pattern in the presence of the pesticide remains the subject of further work and will provide valuable information for the preparation of strains which are highly tolerant to lindane.

 

 

Mutants of Anabaena PCC 7120 lacking of alr1690 and afurA antisense RNA show a pleiotropic phenotype and altered photosynthesis and respiration rates

 

Hernández^1,3, J.A., Alonso¹, I., Maldonado-Rodríguez, R. ², Peleato, M.L. ¹ Strasser, R²., Barja, F. ²and Fillat M,F^1,&.

 

^{1 Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. Universidad de Zaragoza. 50009-Zaragoza, Spain.}

^{2 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.}

^{3 Present address: Plant and Microbial Biology Department. 211 Koshland Hall. University of California at Berkeley. 94720 Berkeley, CA, USA}

 

Abstract

Fur proteins are global regulators present in all prokaryotes. In the cyanobacterium Anabaena sp. PCC 7120 the product of furA controls iron homeostasis and is an essential gene that lays tail-to-tail with alr1690. Recently, it has been identified a new cis-acting antisense RNA, -furA RNA that interferes with FurA expression. The -furA RNA covers the complete furA transcript and it is transcribed in the same message that alr1690 m-RNA. Disruption of the -furA-alr1690 message produces the pleiotropic JAH3 mutant strain that overexpresses FurA. In this work we show that JAH3 cells present altered photosynthetic and respiratory activities and exhibit severe alterations in their ultrastructure. Submitted

 

 

Proceeding, Posters and Oral Presentations in International Meetings and Workshops

 

Poster Leipzig March 2000

Software for calculation and plotting of the membrane energetic fluxes: The pipeline model

 

R. Maldonado Rodriguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Fluorescence Workshop. Institute of Plant Physiology. University of Leipzig

 

 

Oral presentation Basel March 2000

Characterization of six deciduous tree species and four conifer tree species during the growing season 1999 by the JIP test

 

R. Maldonado Rodriguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Swiss Canopy Crane Project Workshop. Institute of Botany, Basel.

 

 

Oral presentation Basel April 2001

Characterization of six deciduous species exposed to elevated CO₂ enrichment during the growing season 2000 by the JIP test

 

R. Maldonado Rodriguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Swiss Canopy Crane Project Workshop. Institute of Botany, Basel.

 

 

Oral presentation Basel March 2003

Characterization of six deciduous species exposed to elevated CO₂ enrichment during the growing season 2001 by the JIP test

 

R. Maldonado Rodriguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Swiss Canopy Crane Project Workshop. Institute of Botany, Basel.

 

 

Oral presentation Basel February 2004

Characterization of six deciduous species exposed to elevated CO₂ enrichment during the growing seasons 2002 and 2003 by the JIP test

 

R. Maldonado Rodriguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Swiss Canopy Crane Project Workshop. Institute of Botany, Basel.

 

 

Poster Zurich April 2001

The JIP Test: A New Tool in Plant Research

 

R. Maldonado Rodríguez, C. Hermans, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Biotechnology Start-up Workshop organized by ETH Zurich

 

 

Poster Bruges June 2001

Biosensing methods to assess environmental stress encountered by sugar beet (Beta vulgaris L.).

 

Hermans, C. Maldonado-Rodríguez R., Strasser, R. J.

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Proceedings of the 24th IIRB Congress. 26-27 June 2001. Bruges, Belgium. Page 424-428

 

 

Poster Australia August 2001

Coral life as probed by their fluorescence emission

 

F. Sinniger, R. Maldonado Rodríguez, R. J. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Link to abstract in CSIRO PUBLISHING - Science Access

Proceedings of International Photosynthesis Congress. Brisbane August 2001

Brisbane Convention & Exhibition Centre, Queensland, Australia, August 18-23, 2001.

Proceedings of the 12th International Congress on Photosynthesis. Brisbane Australia 2001

 

 

 

 

Poster Australia August 2001

Evaluation of the nodulated status of Vigna unguiculata probed by the JIP-test based on the chlorophyll a fluorescence rise

 

P. Schmitz, R. Maldonado Rodríguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Link to abstract in CSIRO PUBLISHING - Science Access

Proceedings of International Photosynthesis Congress. Brisbane August 2001

Brisbane Convention & Exhibition Centre, Queensland, Australia, August 18-23, 2001.

Proceedings of the 12th International Congress on Photosynthesis. Brisbane Australia 2001

 

 

 

Poster Czech Republic, Novy-Hradic August 2001

In vivo Chlorophyll a Fluorescence imaging of herbicide infiltration in Pisum sativum

 

R. Maldonado Rodríguez, R. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

Abstract Book of the II Regional Fluorescence Imaging Workshop. Institute of Photosynthesis Research. Czech Academy of Sciences.

 

 

Oral presentation Varna, Bulgaria September 2002

Luminescent control of biotic and abiotic stress effects in plants

 

Goltsev, V., Zaharieva, I., Lambrev, Maldonado-Rodriguez, R., Strasser, R. J. 

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

European Workshop on Environmental Stress and Sustainable Agriculture, 7-12 September 2002, Varna, 

Bulgaria

 

 

Poster Cordoba July 2002

Análisis De La Respuesta De La Variedad De Olivo “Cornicabra” A Las Aportaciones De Fertilizantes Orgánicos, Mediante El Test De Cinética Rápida De Fluorescencia, Jip-Test

 

Alberto González Moreno¹,  Ronald Maldonado Rodríguez²

^{1 INIA Carretera de la Coruña, 28040 Madrid, Spain}

^{2 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

 

Plant Physiology Workshop Cordoba July 2002 organized by the University of Barcelona, Spain.

 

 

 

Poster Crete September 2002

Artificial Neural Networks for the Characterization of Eight Varieties of Pea (Pisum Sativum)

@ This poster was awarded with the “BEST STUDENT POSTER” prize, selected among other 633 posters.

 

R. Maldonado Rodríguez¹,  A. Gonzales²,  L. Ayerbe²,  J. Sanches², S. V. Pavlov³, R. Strasser¹

^{1 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

^{2 INIA Ctra de la Coruña, 28040 Madrid, Spain}

^{3 Department of Mathematics, University Assen Zlatarov, 8010 Burgas, Bulgaria} 

 

13^th Congress of the Federation of European Societies of Plant Physiology held at Hersonissos, Crete, Greece

 

 

POSTER Crete September 2002

How Pea Plants Respond To Elevated Temperature and Repetitive Saturating Light Pulses

 

A. Okarum, R. Maldonado Rodríguez, R. J. Strasser

Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland.

 

13^th Congress of the Federation of European Societies of Plant Physiology held at Hersonissos, Crete, Greece

 

 

Poster Crete September 2002

Characterization Of Eight Varieties Of Pea (Pisum Sativum) By the Jip-Test

 

A. Gonzales¹, F. Reverchon², R. Maldonado Rodríguez², R. Strasser²

^{1 INIA Ctra de la Coruña, 28040 Madrid, Spain}

^{2 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

 

13^th Congress of the Federation of European Societies of Plant Physiology held at Hersonissos, Crete, Greece

 

 

Poster EMEC3 Geneva December 2002

Neural Networks for Monitoring Environmental Stress Effects on plants

 

Ronald Maldonado Rodríguez¹, Stancho Vaelkanov Pavlov², Reto Strasser¹

^{1 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

^{2 Department of Mathematics, Technological University Assen Zlatarov, 1 Prof. Jakimov blvd, 8010 Burgas, Bulgaria} 

 

3rd European Meeting on Environmental Chemistry EMEC3, December 11 to 17 2002 Geneva, Switzerland. 

http://www.u-bourgogne.fr/ACE

 

 

Poster EMEC3 Geneva December 2002

Self-Organizing Map (SOM) for monitoring the evolution of Rhizobium nodulation status in Vigna unguiculata

 

P. Schmitz¹­, R. Maldonado Rodríguez¹, S. Pavlov², W. Broughton³, R. Strasser¹

^{1 Laboratory of Bioenergetics and Microbiology. University of Geneva. 10, Chemin des Embrouchis, CH-1254 Jussy, Geneva, Switzerland}

^{2 Department of Mathematics, Technological University Assen Zlatarov, 8010 Burgas, Bulgaria} 

 

3rd European Meeting on Environmental Chemistry EMEC3, December 11 to 17 2002 Geneva, Switzerland.

http://www.u-bourgogne.fr/ACE

 

 

Poster Geneva EMEC3 December 2002

A fast routine for monitoring plant health status

 

A. Okarom, R. Maldonado Rodríguez, S Elmadidi & R. J. Strasser

 

3rd European Meeting on Environmental Chemistry EMEC3, December 11 to 17 2002 Geneva, Switzerland. 

http://www.u-bourgogne.fr/ACE

 

 

Oral presentation Cordoba March 2003

A comparison of the JIP Test and the Self-Organizing Map neural network for the classification of pea varieties into Drought Stress Resistance categories

 

Ronald Maldonado Rodríguez¹, Stancho Pavlov², Reto Strasser¹

(1)  Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

(2)  Department of Mathematics, University Asen Zlatarov, 8010 Burgas, Bulgaria

 

University of Cordoba, March 11 2003. Seminar organized by the Forest Sciences Faculty of the University of Cordoba, Spain.

 

 

Oral presentation Mallorca March 2003

Can machines recognize stress in plants? A neural Network Approach

 

Ronald Maldonado Rodríguez¹, Stancho Pavlov², Reto Strasser¹

(1)  Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

(2)  Department of Mathematics, University Asen Zlatarov, 8010 Burgas, Bulgaria

 

Congress of the Association of Applied Biologist “Optimisation of water use by plants in the Mediterranean” held at Cala Bona, Majorca, from 24 to 27 March 2003-11-27

http://www.aab.org.uk

 

 

 

Poster presentation Mallorca March 2003

Effect of water stress and re-watering of eleven varieties barley in early stage: Characterisation by the JIP-test 

 

R Maldonado-Rodriguez, A Okarom (University Of Geneva, Switzerland), S Elmadidi (Laboratoire D'etude De La Variabilité Génétique, Morocco), A Gonzales (Inia, Spain) & R J Strasser (University Of Geneva, Switzerland)

 

Congress of the Association of Applied Biologist “Optimization of water use by plants in the Mediterranean” held at Cala Bona, Majorca, from 24 to 27 March 2003-11-27

http://www.aab.org.uk

 

 

Poster Mallorca March 2003

Response of lentil plant to drought stress combined with short time exposure to high temperature shocks

 

A OKAROM, R MALDONADO-RODRIGUEZ (University of Geneva, Switzerland), S ELMADIDI (Laboratoire d'Etude de la Variabilité Génétique, Morocco) & R J STRASSER (University of Geneva, Switzerland) 

 

Congress of the Association of Applied Biologist “Optimization of water use by plants in the Mediterranean” held at Cala Bona, Majorca, from 24 to 27 March 2003-11-27

http://www.aab.org.uk

 

 

Oral presentation Basel February 2004

Chlorophyll a fluorescence patterns of 6 deciduous species exposed to normal and elevated CO2 in an open air FACE installation

 

R. Maldonado Rodríguez, R. Strasser

Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

 

Swiss Canopy Crane Project Workshop February 2004. Institute of Botany, Basel.

 

 

Poster Barcelona March 2004

CHLOROPHYLL FLUORESCENCE IN FIVE PROVENANCE Pinus halepensis Mill TO QUANTIFY RESPONSE TO WATER STRESS

 

Rafael Mª Navarro Cerrillo, Ronald Maldonado Rodríguez, David Ariza

Bioenergetics Laboratory, 10 Chemin des Embrouchis, CH-1254 Jussy, Switzerland

 

Poster Lisbon July 2005

A Methodological Approach for Pattern Recognition System using discriminate analysis and artificial neural networks

 

Anna Pérez-Méndez, Elizabeth Torres-Rivas,  Francklin Rivas-Echeverría, Ronald Maldonado-Rodríguez

 

WSEAS Neural Networks, Fuzzy Systems, Evolutionary Computing Conference (Lisbon 2005)

 

 

Poster Presentation. Bern 4 April 2007

Substituting the former dinosaurs by trees reverts global warming and climate changes

 

Ronald MALDONADO RODRIGUEZ¹, Yuxin YUAN², Reto J. STRASSER³

1.     Institute Universitaire d’Etudes du Développement, 63 Rue de Lausanne, Genève 1202, Suisse. e-mail: [email protected]. http://www.geocities.com/fluoromatics

2.     College of Forestry, Agricultural University of Hebei, Baoding 071000  Hebei, P.R. China. E-mail: [email protected] http://www.hebau.edu.cn

3.     Laboratory of Bioenergetics, University of Geneva, 10 Ch. des Embrouchis, Jussy, Geneva CH-1254, Switzerland. email: [email protected]  http://www.unige.ch/sciences/biologie/bioen

 

Eighth Swiss Global Change Day. Bern 4 April 2007

 

 

 

Magazine cover page designed by Ronald Maldonado Rodriguez

 

I love drawing and painting. All the graphic design included in the software that I have written has been done entirely by me. I have drawn some cover page designs for four serious scientific journals. For having a look at one of these covers please follow the links below:

Functional Plant Biology

Plant Biology 5(3):315-323, 2003 May

 

 

Cited in Scientific Journals

 

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1469-8137.2005.01385.x

 

 

Web sites created by Ronald Maldonado Rodriguez

·         The Bioenergetics Laboratory of the University of Geneva. Link here

·         The Microbiology Laboratory of the Plant Biology department at the University of Geneva. Link here

·         Chlorophyll Fluorescence and Fluoromatics web page at Geocities. Link Here

 

Ronald Maldonado Rodriguez on the NEWS

 

Baillod, P. M. & Martini, G. O. Environmental assays by photosynthetic fluorescence. Bioworld 2, 2001. www.bioworld.ch. ISSN 1424-8514.

 

The APTE (Association for the Application and Promotion of Electronic Technologies) published an article in the BioWorld Europe Magazine about the work we do at the Bioenergetics Laboratory of the University of Geneva, Switzerland. You can read it in English or French. It is quite interesting and very informative.

 

See my Publications

See My Scientific Interests

See My PhD thesis progress report

See Software that I have written

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