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The cDNAs sequenced ranged in its starting point and length in the whole sequence. In WRKY1, all cDNA segments started before point 3, indicating that the domains ��LVATYEGEHNH�� and ��RRNLAEQMAMTLTRDPSFKAALVTAL�� maybe required for heterodimerization. On the other hand, the coiled coil domain ��KKDPEVKVAAL EAELKRMGAENRQLSEML�� and the WRKY domain may not be vital to the interactions, since the shortest interactors identified only contained the two conserved domains described above.
Beta- galactosidase catalyzes substrate and produces a blue color. Colorless colonies are presumed to be false interactors that survived on negative histidine plates for unknown reasons. However, there were no false positives in this screen
Plate with interacting colonies �V only interacting colonies with activated histidine reporter genes can survive on negative histidine plates.
SDS gel electrophoreses allows for the identification of different interactors by observing the DNA band sizes. Out of the cDNAs that appeared to contain the same cDNA, only one or two were sequenced. The smaller the fragment, the further and faster it travels towards the negative pole. Lane identity (in order): DNA marker, 23c-1, 23c-2, 24c-1, 24c-2, 25c-1, 25c-2, empty lane, 26c-1, 26c-2, 27c-1, 27c-2, 29c-1, 29c-2, 31c-1, 31c-2, 33c-1, 33c-2, 34c-2, 34c-1, 36c-1, 36c-2, 35c-1, 35c-2, 37c-1, 37c-2, 39c-1, 39c-2, 40c-1, 40c-2, 41c-1, 41c-2, 3m-1, 3m-2, DNA marker. Xbi-2m-2, xbi-1c-1, xbi-2c-1, xbi-3c-2, xbi-4c-1, xbi-5c-1, xbi-6c-1, xbi-8c-1, xbi-9c-1, xbi-10c-1, xbi-11c-1, xbi-12c-1, xbi-14c-1, xbi-18c-1, xbi-19c-1, xbi-20c-1, xbi-22c-1 were selected for sequencing. [Colonial designations reflected the order in which they were extracted from the plate �V xbi (interactor obtained from Xb10 screen) �V 1 (order in which colony was picked) c/m (indication of library, C64 or Moroberekan) -2 (two colonies were picked from each plate and placed on liquid LB amp media (see Isolation of Plasmid DNA from Yeast in the methods section.)) (xbi-1c-2.) (To abbreviate, the xbi part will be omitted. (xbi-5c-1=5c-1.))]
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