Tulsa and Oklahoma City have the only NAB sampling stations for the entire state of Oklahoma. This study attempts to see how well the pollen concentrations in the metropolitan area of Tulsa relate to a nearby rural area of Cushing, OK. This study examined spatial variability of pollen concentrations from two Burkard spore traps located approximately 85 km (53 miles) apart, with one located 12 m above ground level in the metropolitan area of Tulsa, and the other located 1.5 m above ground level in a rural area approximately 85 km west of Tulsa. Spring tree pollen and fall weed pollen collected in the year 2001 from the two locations were examined for the types of pollen taxa present, as well as the pollen concentrations for those taxa. Mean pollen concentrations for the spring and fall seasons were both higher for Tulsa than those for Cushing; however, the means were not significantly different. Further examination showed that Tulsa pollen was represented by 19 tree taxa in the spring and 10 weed taxa in the fall, while the Cushing atmosphere consisted of 17 tree taxa and 7 weed taxa. Finally, the spring atmosphere in Tulsa was dominated by Quercus pollen, while the same time period in Cushing was dominated by Cupressaceae. While the means for the total pollen concentrations from the two locations were not significantly different, the pollen load in the atmosphere from each location is indicative of two moderately different biotic regions with distinct allergy symptom inducing plants.