Assignment VI

 

Part  I
 

Neuraminidase (NA) is selected sequence for primer design because genetic variation of NA gene resulting in the emergence of new influenza virus strains has frequently been recognized.

Neuraminidase gene were isolated from human who live in 2 location of Thailand.

1.Kanchanaburi province>gi|46578135|gb|AY555151.2| Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) neuraminidase gene, partial cds

2. Supunburi >gi|46360356|gb|AY577315.1| Influenza A virus (A/Thailand/3(SP-83)/2004(H5N1)) neuraminidase gene, complete cds

3. Supunburi >gi|46360358|gb|AY577316.1| Influenza A virus (A/Thailand/4(SP-528)/2004(H5N1)) neuraminidase gene, complete cds
 
 
 
Part II Primer design (click)
 
 
Part III  Relationship (click)
 
 
Part IV Software employment
 

The tools which were employed in this assignment
 
1. Nucleotide search from Genbank NCBI website
2. Consensus sequence finding Bioedit
3. Primer and probe design Primer3 (web-based)
4. Primer and probe verification Blastn (NCBI website)
5. Multiple alignment and Phylogenic construction Bioedit (Accessory application function) which provided ClustalW alignment, DNA Maximum Likelihood program with molecular clock function parameters
 

Created by

MISS PATIYA PHENG-ON

4737611 SCFS/M

Forensic Science Grad.

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