Assignment VI

Part II Primer design

 

Consensus sequence finding (Bioedit)

Input sequence

 

 

The result of Consensus region finding.

 

 

Nucleotide composition finding. (GC content = 42.40%)

 

 

Primer design (Primer3-web based)
 

Input consensus sequence which is the result from Bioedit program.

 

 

 

Condition setting

 

The result was shown after clicked "Pick Primers" button.

 

Result

 

Conclusion

The result from primer design, software had selected one set for forward, reverse primer and oligonucleotides probe described down here;

Product size is 203 basepairs.

Position Length Tm Sequence
Left primer 989 20 56.96 GTGTCCTCTAACGGGGCATA
Right primer 1191 20 60.05 CTCCCGCTATATCCTGACCA
Probe 1049 20 60.05 TGGATCGGGAGAACAAAAAG

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