PATH 711
Techniques in Pathology 3(1-2)
Learning Objectives:
Students will learn the requirements to work in Pathology lab and the
concepts of various techniques used in routine, concepts about PCR/ELISA
etc. and their uses in disease diagnosis and handling of molecular data and
its analysis/interpretation.
Theory:
Pathology laboratory safety guidel ines/protocols; Introduction to biosafety;
Principles and uses of electrophoresis, histochemistry,
immuno-histochemistry, ELISA, PCR, RT-PCR, real time PCR. Image analysis
software(s); Disease and PCR data entry, handling and analysis; High
Performance Liquid Chromatography.
Practical:
Centrifugation; Microscopy; Gross a nd microscopic digital photography and
image analysis; Micrometry; Measuring oxidant status; Spectrophotometry;
Histopathology and immunohistochemistry; Electrophoresis; Cell membrane
permeability; ELISA; Apoptotic cell staining; RBC membrane rigidity/viscoelasticity
t e s t i n g ; H ypo-osmotic swelling (HOS) test; Somatic cell count; PCR,
RT-PCR, Isothermal PCR; Extraction of DNA from tissues and blood cells;
Computer assisted DNA sequence data interpretation.
Suggested Readings:
1. Coles, E.H. 1998. Veterinary Clinical Pathology. 5th Ed. W.B. Saunders
Co., Philadelphia, PA, USA
2. Electronic sources – digital libraries, books, journals, etc.
3. Kaneko, J.J. , J. W. H arvey and M.L. Bruss (eds.). 1997. Clinical
Biochemistry of Domestic Animals. 5th Ed. Academic Press, New York, USA.
4. Rosenfeld, A.J. and S.M. Dial. 2010. Clinical Pathology for the
Veterinary Team. 1st Ed. Blackwell Pub Professional, Ames, IA, USA.
5. Stockham, S.L. and M.A. Scott. 2009. Fundamentals of Veterinary Cl inica
l Pa thology. 2nd Ed. Blackwell Pub Professional, Ames, IA, USA