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| WRKY Factors and their Role in the Signal Transduction Pathway From the sequences of the initial 17 cDNAs and the 6 after PCR amplification analysis, three distinct types of interactors were isolated �V WRKY1, WRKY2, and WRKY. WRKY proteins have a commonly conserved zinc-finger type WRKY domain (amino acid sequence order) and are found exclusively in plants. These proteins were shown to bind specifically to W Box-type [(C/T)TGAC(C/T)] DNA sequence elements both in vitro and in vivo (Du L., 2000).Studies also show that the W box is the most common element found in defense gene promoters (Du L., 2000). The plant-specific existence of this super family suggests that WRKY factors are involved in several unique processes required for plant development and/or for the integration of environmental cues. They also appear to play an important regulatory role under stress-related conditions, particularly in innate immunity responses. Approximately 80 WRKY��s have been identified in rice, although their function has not be thoroughly studied (Du L., 2000). |
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