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Fig. 5.
Reduction in shunt resistance induced by
current injection simultaneous to intracellular perfusion with fluoride ions. (a, b) Human atrial myocyte placement prior
and after its placement inside the hole of a perfusion tube with suction (see
Fig. 3).
(c) Superimposed storage-oscilloscope traces
of membrane potential recorded during current injection of 100 nA and tubing
perfusion with buffered 150 mM CsF (see Bustamante and McDonald, 1983). The uppermost and lowermost traces
correspond, respectively, to the initial and final (steady-state) levels of the
membrane potential of �12 and �102 mV.
From these observations can be concluded that Rshunt was
about 0.1 and 1 MW. Without F-
inside the tubing, the higher value of Rshunt was not attained
(Bustamante, 1981). Experiment
81070401.