28/4/05

  1. 2002_Science_Miller_twophoton_intact_lymph_node.pdf

27/4/05

  1. 2000_SingleMolecule_Schutz.pdf 3D Imaging of Individual Ion Channels in Live Cells at 40 nm Resolution. Full of Cy-dye goodness!

26/4/05

  1. 2004_MicroscopyResTech_Esposito_twophoton.pdf
  2. 2000_BioEssays_Whitaker_gfptagged.pdf

25/4/05

  1. 2005_TIBTech_Cavagnero.pdf - A good paper on FIAsH - a visiualisation technique which uses As tags to go anywhere a protein can go, and is quicker to light up recently folded protein than GFP tags. We'd better know all about this by question time.

22/4/05

  1. 2000_JournMicroscopy_Wiseman_twophoton.pdf
  2. 2004_MicroscopyResTechnique_Tung_multiphoton.pdf
  3. 2005_JournFluorescence_Buehler_multiphoton.pdf
  1. 2004_CurrOpImmuno_Bryant.pdf
  2. 2004_Immunity_Bousso_TwoPhoton.pdf We all need to be familar with 2photon. Paul is very enthusiastic that we are up with the multiphoton stuff.
  3. 2004_Nature_Boes.pdf
  4. 2004_TICB_Boes.pdf
  5. 2005_TrendsInImmuno_Chow.pdf
  6. 2003_Cell_Vale.pdf
  7. 2003_PNAS_Murk.pdf
  8. 2004_JournalofImmuno_Poloso.pdf
  1. 2002_PNAS_Sonnleitner_TIF_microscopy.pdf
  2. 2001_PNAS_Ajo-Franklin_TIF_Microscopy.pdf

20/4/05

  1. 2002_Nature_Boes.pdf This is the paper that made the cover of nature in 2002. It has really nice pictures of dendritic cells, and talks about the groundbreaking technique of expressing MHC II with a GFP tag.
  2. 2002_Nature_Chow.pdf Uses different microscopy techniques - including TIR-FM. Shows that on stimulation, the microtubule "tramtracks" extend from the lysosome to the macrophage plasma membrane.
  3. 2003_Nat_Rev_Immuno_Honey.pdf Excellent review article. Covers MHC II expression and transport, and compares it to CD1. Also has a history of MHC II research in a little box. No actual imaging stuff in this paper - the imaging stuff is in the papers it references.
  4. 2004_TICB_Boes.pdf just down load it. It's boes, so it's good. If you really want to know, Boes fed some macrophages some glow in the dark peptides and then observed bi-directional transport of the peptide within vesicles. That's the proof for our current MHC II loading model as described in lectures.
  5. 1998_Nature_Ridge.pdf An early paper proposing that dendritic cells activate T-cells.

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