Group D's MHC Project

Group D's Topic is: Imaging Techniques used to track transport of MHC class II molecules in dendritic cells.

Group D is:

and member emeritus

News

28/4/05: Tim has uploaded his slides!

This website is full. moving to http://www.geocities.com/mhcproject2/ for all future updates

28/4/05: Chris has uploaded his slides! chris1.ppt Yay!

28/4/05: More webspace can be found at http://www.geocities.com/mhcproject2. Password is same as this site

28/4/05: Tim has found a great paper on 2photon being used to get a 3d image of an intact lymph node. It's in the papers section. Also, what do you guys think of FLIP and FRAP as described in yesterday's dep seminar?

27/4/05: Group D meeting with Paul is Thursday 28th April at 4:45pm at his office. 27/4/05: Jin just uploaded a paper on ion channel visualisation with 40nm resolution. Think it would allow the visualisation of different classes of antigen presenting molecules in the same experiment? Get it while it's hot!

26/4/05: Vanessa has found two excellent papers, and is closing in on finishing her slides! papers

25/4/05: Chris has found an excellent paper on FIAsH which is in the papers. The As is for Arsenic!

23/4/05: Microstructure of oil shale imaged with confocal http://geolmag.geoscienceworld.org/cgi/content/full/140/4/469

23/4/05: Jin found a great introduction to multiphoton, including the section called "multiphoton vs confocal" http://www.aecom.yu.edu/aif/intravital_imaging/introduction.htm 23/4/05: How's your French? Full text article on FRAP, FLIP, FRET, BRET, FLIM, PRIM...new techniques for a colourful life from france http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15525500

22/4/05: More of Lauren's papers have been uploaded! Thanks Lauren. Some very detailed articles about the future of immunological imaging with multiphoton in the papers section

22/4/05: Half of Lauren's papers have been uploaded. Thanks Lauren! We are going to be grilled on Multiphoton Microscopy in QT, so we need to read that Bousso paper from Immunity. Also Chris has found a paper on FlAsH technique. Uploading...

22/4/05: papers on TIF Microscopy added to the papers section.

22/4/05: Got a simposium abstract that mentions Total Internal reFlection Microscopy here http://www.nature.com/horizon/livingfrontier/sessionsummaries.html

22/4/05: Minsky's homepage has moved. His new home for the abstract of how he invented the confocal microscope specifically to image the connections between neurons in 1955 is here http://web.media.mit.edu/~minsky/papers/ConfocalMemoir.html and his academic biography is here http://web.media.mit.edu/~minsky/minskybiog.html

22/4/05: Here is a page that claims that the confocal microscope was invented by legendary MIT hacker Marvin Minsky. http://web.ncifcrf.gov/rtp/ial/cml/details.asp. They're from the government. Trust them?

22/4/05: Jin Found an alternative imaging technique - 4Pi. It's a new kind of confocal microscopy that can image living tissue focusing on ~100nm thick layers at a time and then uses computer imagery to assemble the layers into a 3d structure. http://www.4pi.de/ has links to nature papers on 4Pi, and Leica sells a commercial instrument.

22/4/05: Jin Found a useful page on Confocal microscopy http://www.physics.emory.edu/~weeks/confocal/

21/4/05: Project roles assigned:

  1. Introduction - Signpost Structure of the talk / why image MHCs? - Tim Ryan
  2. Confocal Microscopy - history and practice - Vanessa Wall
  3. Alternative imaging techniques - 2 photon, others - Lauren Webb
  4. Alternative imaging techniques - tracking movement without genetic engineered fluoro tags... Cryo-EM time points and their abandonment? others. - Chris Harrison
  5. Summary - Physiological significance - how we judge a good imaging technique - What, exactly do we want to see - future directions - Jin Kee

20/4/05: Urgent. Need to get on web of science and look for papers on confocal microscopy that reference Boes 2002, and are referenced by Boes 2002.

20/4/05: Jin Uploaded some papers

19/4/05: Got some papers.

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