1. Polk Museum of Art, Lakeland, FL is a small but interesting museums which has exhibits for local student art (elementary through college) as well as famous exhibitions.
2. Salvador Dali Museum, St Petersburg, FL exhibits works of the famous Dali and has collections which change so that repeat visits bring new insights to his well know talents.
3. Charles Hosmer Morse Museum Of American Art has the world's most comprehensive collection of the works of Louis Comfort Tiffany, as well as a major collection of American art pottery.
4. MOSI/Museum of Science and Industry, Tampa, FL has an IMAX Dome Theatre, a Planetarium, and a Kid's in Charge exhibit for children 12 and under. This museum is very large, and has many interesting science exhibits, fun for the entire family.
5. Orlando Museum of Art, Orlando, FL has exhibits which change every few months, or remain for a bit longer, and they have a wide variety of taste, from Aztec Art to Motorcycle Art.
6. Albin Polasek Museum and Sculpture Gardens has outdoor exhibits of Polasek's sculptures, and also, when in season, exhibits highlighting different types of plants and flowers.
7. Florida Gulf Coast Railroad Museum, Manatee County, FL is a great museum with actual restored railcars circa the early 20th century, with original lighting fixtures, and everything. There are train rides on the weekend rain or shine!
8. The John and Mable Ringling Museum of Art, Sarasota, FL has a great selection of art from all over the world and also a waterfront estate with a rose garden.