Subjects studied:
BioAnalytical Science 3
Cell Biology
Analytical Chemistry
General Microbiology
Molecular Biology
BioAnalytical Science 3 (Jan - Mar 2000)
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  1. Spectrophotometry
  2. Enzyme Linked Immunosorbant Assay (ELISA)
  3. Enzymatic Glucose determination ( Glucose oxidase, Peroxidase)
  4. Serum Protein Separation by cellulose acetate electrophoresis
  5. Determination of glycated haemoglobin using an affinity resin kit.
  6. Gel filtration of fluorescent antibodies
  7. Lactate dehydrogenase assay


Cell Biology (Sept.-Dec 1999)

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  1. Introduction to Biosafety
  2. Use of the Microscope
  3. Use of the Haemocytometer
  1. Basic aseptic technique
  2. Making %quotBHK$quot medium. (based on GMEM)
  3. Passing BHK-21 cells
  1. Virus (TMEV GD7) infection of BHK cells
  2. Setting up cells for virus plaque assay
  1. Virus assay
  1. Cryopreservation of BHK cells
  2. Thawing and recovery of cells
  3. 1.Agglutination of Bacteria
  1. Precipitation in a gel.


Analytical Chemistry (Sept – Dec 1999)

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  1. Determination of volatile trace components in Whiskey samples by Gas Chromatography.
  2. Determination of vanillin in vanilla essence by Gas Chromatography.
  3. Schimadzu High Performance Liquid Chromatography (HPLC) Experiment.
  4. Fluorimetric analysis of Quinine in Tonic Water.
  5. Extraction and ultraviolet determination of carotenoids in Tomato purée.
  6. Determination of alkali metals by Flame Photometry.
  7. Determination of Iron in Cornflakes by Atomic Absorption Spectrophotometry.
  8. Karl Fischer Titrations of Vodka and Whiskey using the DL35 Titrator
  9. Use of Radiometer Autotitrator.


Microbiology. (Sept – Dec 1999)

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  1. Agar Plate Methods.
  2. Enumeration of Faecal coliforms. (MPN)
  3. Presence or Absence test, Enumeration by plate count, and confirmation of Enterobacteriaceae.
  4. Enumeration of presumptive Enterococci.
  5. Isolation and Identification of yeasts and moulds.
  6. Isolation and Identification of Staphylococcus aureus.
  7. Isolation and Identification of Salmonella species
  8. Isolation and Identification of Listeria species
  9. Isolation and Identification of Pseudomonas species


Molecular Biology. (Sept – Dec 1999)

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  1. Restriction endonuclease digest of bacteriophage lambda DNA.
  2. DNA Isolation from Gram Negative Bacteria and Polymerase Chain Reation (PCR) based Ribotyping.
  3. DNA Isolation from Human blood and Random Amplified Ploymorphic DNA based PCR.
  4. Southren Blotting Molecular Analysis using probe hybridization. (DIG-labelled PCR product)
  5. Transformation of Escherichia coli with recombinant plasmid DNA.
  6. Rapid colony screening of cloned bacteria colonies for presence of plasmid vector pCR 2.1
  7. Restriction endonuclease digestion of M13 PCR products to illustrate the presence of a ligated PCR product.
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