The International School of Panama
Panama City, Panama
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Soil and Terrestrial Habitat

Biological Analysis of Soil
Table 1: Texture and Consistency of Soil

The Texture and Consistency of Soil
Sample Source Texture Consistency Dry / Wet
1 Riverbed Loamy Sand Crumbly, dry, hard, med. Particles / grainy, rough, crumbly
2 Field (25 m from river) Silky Clay Loam Soft, small- med particles, crumbly, thin / squishy, holds together, soft, particles are barely visible
3 Forest (50 m from river Silky Clay Loam Soft, small particles, crumbly, very powdery / mushy, soft, malleable, particles not visible.

Water Content of Soil
Table 2: Water Content of Soil

Water Content in Soil Samples
Object Weighed Weight (in grams)
  Riverbed  Field (25m) Forest (50m)
Empty Beaker 51.78 179.09 169.09
Cup plus Fresh Soils 134.1 233.92 215.32
Cup plus Dry Soils
111.8
192.86
207.1
Water Lost
22.3
41.66
8.22
Percent H20
27.1
76
17.8

Organic/Ash Content in Soil
Table 3: Organic/Ash Content in Soil

Weighed Organic/Ash Content

Sample Weight (g)
Riverbed
60.06
Field (25m)
13.17
Forest (50m)
38.01

Bulk Density
Table 4: Bulk Density of Soil

 
Riverbed
Field (25m) Forest (50m)
Volume of 70g 52 mL 69.8 mL 77.7 mL
Bulk Density 1.35 g/mL 1.002 g/mL 0.90 g/mL

Water-holding Capacity and Capillary Action
Table 5: Water-holding Capacity and Capillary Action

 
Riverbed
Field (25m) Forest (50m)
Weight of Empty Column 3.68 g 3.59 g 3.59 g
Weight of Dry Soil and Column 20.53 g 15.24 g 14.44 g
Weight of Dry Soil 16.85 g 11.65 g 10.85 g
Weight of Saturated Soil and Column 28.24 g 23.66 g 22.03 g
Weight of Water 11.39 g 2.01 g 11.18 g
Water-holding Capacity 0.889 g/mL 0.938 g/mL 0.873 g/mL
Distance Traveled 70.2 mm 69.3 mm 67.4 mm
Time 1.57 min/ 117 sec 22.22 min/ 1342 sec 13.25 min/ 805 sec
Rate 44.71mm/min

0.745 mm/sec

3.12 mm/ min

.052 mm/sec

5.08 mm/min

.084 mm/sec

Permeability
Table 6: Soil Permeability

Permeability in Different Sample Soils
 
Dry
Wet
Sample Time for 1st Drop (s) Time for Entire Sample (s) Time for 1st drop (s) Time for Entire Sample (s)
Riverbed 43.27 139.36 57.53 707.43
Field (25m) 1548 2165 961 8109
Forest (50m) 849 1480.5 128 13203.36

Slope
Table 7: Slope

Y1 and Y2 for % Slope of the Areas
Sample Area Y-Component Measurement (cm)  Measurement (ft)
Riverbed Area Y1 148.3 4.94
  Y2 230 7.67
Field Area (25m) Y1 52.7 1.76
  Y2 83 2.77
Forest Area (50m) Y1 47.4 1.58
  Y2 81 2.7

pH of Fresh Water

Type of water PH # given by pH paper pH # given by the pH kit
Fresh water #1 (top layer) 6.5 7.4
#1  (middle layer) 6.3 7.3
Fresh water #2 (top layer) 6.4 7.3
#2 (middle layer) 6.2 7.2
Rain Water 6 5.6

Total Solids of Fresh Water

Type of water Mass of beaker Mass of beaker with solids Total solids
Fresh water #1 (top layer) 68.30g 68.33g 300 mg/L
#1 (middle layer) 67.89g 67.92g 300 mg/L
Fresh water #2 (top layer) 67.41g 67.43g 200 mg/L
#2 (middle layer) 69.30g 69.33g 300 mg/L

Fecal Coliform of Fresh Water

Type of water Fecal coliform
Fresh water #1 (top layer) Not present
#1 (middle layer) Not present
Fresh water #2 (top layer) Not present
# 2 (middle layer) Not present

Water Content

Testing for Nitrates

The measure of human and animal waste, decomposing organic matter, and fertilizer runoff.  
Downstream 0
Upstream 0
Testing for Phosphates
The measure of plant and animal wastes, and industrial detergent waste water  
Downstream 0.2 ppm
Upstream 0.3ppm

 

Testing for Turbidity
The measure of the relative cloudiness in water 
  number of additions amount in mL final measure in JTU's
Downstream 1 0.5 5
Upstream 2 1 10
Testing for Hardness
The amount of calcium and magnesium in the water.
  total in ppm calcium in ppm magnesium in ppm
Downstream 60 40 20

 

                               Testing for Dissolved Oxygen 

the measure of the amount of oxygen that has been absorbed into the water 
  total amount in ppm
Downstream 7.4
Upstream 6
Current Velocity
Velocity Upstream 0.425 +- 0.0841 m/s
Velocity Downstream 0.627 +- 0.075 m/s
Refractometer
  total in ppm
Downstream 0
Upstream 0

Aquatic Productivity

Primary Productivity in an ecosystem may be calculated by measuring the rates of oxygen production and consumption in order to estimate the  rate of photosynthetic carbon fixation.

This can be done by using the �light and dark bottle� method

The difference Between the initial oxygen measurements and the dark bottle oxygen measurement indicates amount of oxygen consumed by respiration of the organisms in the bottle.

Therefore, the respiration rate is calculated by:

R= (Initial O2 - O2 in dark bottle) / time (d)

The difference between the initial oxygen measurement and the light bottle oxygen measurement indicates the net primary production.

Gross  productivity of O2 ( does not take account losses) is:

(O2 in light bottle - O2 in dark bottle)/ time (d)

Therefore, net primary productivity is :

Gross productivity � respiration rate

Results ( using Winkler Method):

Initial O2 = 7.2 ppm

Dark Bottle O2= 6 ppm

Light Bottle O2= 8 ppm

Accepted value of Dissolved Oxygen in 23�C= 8.2 ppm

This means that river tested is a healthy and stable ecosystem capable of supporting different kinds of organisms.

Productivity Results:

Respiration Rate of O2 = 1.52 ppm/d

Gross Productivity of O2 = 2.53 ppm/d

Net Primary Productivity of O2 = 1.01 ppm/d

The experiment was successful and this ecosystem works efficiently as it has a productivity as 1.01ppm per day.

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