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PLATELET AND NEUTROPHIL ANTIGEN

                  Table of Contents

        I.       Introduction

       II.       Discussion

     Clinical condition related to platelet and neutrophil antigen.

     a.     Immune Platelet Destruction

     b.     Post-transfusion (Isoimmune) Purpura

     c.      Neonatal Isoimmune Thrombocytopenia

     d.      Neonatal Isoimmune Neutrophenia

     Fcgamma receptor mediated cellular function

     Polymerase Chain Reaction (PRC)

     How to do neutrophil and platelet genotyping

     a.      Method

     b.      Blood Donors

     c.       Establishment of B-Lymphoblastoid Cell Lines (B-LCLs)

     d.      Isolation of genomic DNA from whole blood and B-LCLs

     e.      Genotyphing of HPA-1 to HPA13 by restricted fragment length polymorphism

     f.       Genotyping of Na1, NA2 and SH by allele-specific PCR

     g.       Result

     III.       Conclusion

     IV.        Reference