III. CONCLUSION

                      The inexhaustible source of reference DNA derived from B-LCLs allowed the evaluation of reliable HPA and HNA genotyping for quality control purposes it should facilitate the development of DNA typing in blood centers and clinical laboratories.

                      Several techniques for HPA and HNA genotyping exist. Currently, genotyping based on PCR-SSP is widely used. However, reference DNA derived from homozygous, phenotyped individuals is required to evaluate the specificity of the allele-specific primer used for PCR amplification. Commonly, genomic DNA derived from peripheral blood cells of typed individuals is used as reference DNA. However, such reference DNA is difficult to obtain, particularly from individuals carrying rare alloantigens.