Protein

 

The protein coded for by the GRHPR gene is CAI13848 glyoxylate reductase/hydroxypyruvate reductase [Homo sapiens] (328 aa)7, it can also be found as AAD465178.  After doing a Conserved Domain Search the following domains were found similar to the initial amino acid sequence entered into the BLAST search.  See the chart below.

Domains Related to Initial Amino Acid Sequence Search

Domain Function
2-Hacid_dh_C Inserted into the catalytic domain
2-Hacid_dh Largest portion of the catalytic domain of 2-hydroxyacid dehydrogenases
GlutR_NAD_bind Uses NADPH as a cofactor
LdhA Energy production and conversion
SerA Amino acid transport and metabolism
MmsB Lipid metabolism
HemA Coenzyme metabolism
TyrA Amino acid transport and metabolism
ProC Amino acid transport and metabolism

 

Protein Structure

The protein structure of GRHPR is not known.  By doing a search through the NCBI related structure database, I received the following results.  The closest protein with a known 3-D structure is: 1GDH Chain B, D-Glycerate Dehydrogenase (Apo Form) (E.C.1.1.1.29).  When comparing the two amino acid chains, the following results were displayed:

Score (bits) = 168 E_value = 2e-46
Aligned Length = 307 Sequence Identity = 35 % 
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|
gi 5669919   8 KVFVTRRIPaegRVALARAADC-EVEQWDSDEPIPAKELERGVAGAHGLLCLLSDHVDKR 66
1GDH_B       3 KILITWPLP---EAAMARARESyDVIAHGDDPKITIDEMIETAKSVDALLITLNEKCRKE 59

                       70        80        90       100       110       120
               ....*....|....*....|....*....|....*....|....*....|....*....|
gi 5669919  67 ILDAAGANLKVISTMSVGIDHLALDEIKKRGIRVGYTPDVLTDTTAELAVSLLLTTCRRL 126
1GDH_B      60 VIDRIPENIKCISTYSIGFDHIDLDACKARGIKVGNAPHGVTVATAEIAMLLLLGSARRA 119

                      130       140       150       160       170       180
               ....*....|....*....|....*....|....*....|....*....|....*....|
gi 5669919 127 PEAIEEVKNGGWTSWKPLWLCGYGLTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQ 186
1GDH_B     120 GEGEKMIRTRSWPGWEPLELVGEKLDNKTLGIYGFGSIGQALAKRAQGFDMDIDYFDTHR 179

                      190       200       210       220       230       240
               ....*....|....*....|....*....|....*....|....*....|....*....|
gi 5669919 187 PRPEEAAEFQAEF-VSTPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISR 245
1GDH_B     180 ASSSDEASYQATFhDSLDSLLSVSQFFSLNAPSTPETRYFFNKATIKSLPQGAIVVNTAR 239

                      250       260       270       280       290       300
               ....*....|....*....|....*....|....*....|....*....|....*....|
gi 5669919 246 GDVVNQDDLYQALASGKIAAAGLDVTSPEPlPTNHPLLTLKNCVILPHIGSATHRTRNTM 305
1GDH_B     240 GDLVDNELVVAALEAGRLAYAGFDVFAGEP-NINEGYYDLPNTFLFPHIGSAATQAREDM 298

                      310
               ....*....|....
gi 5669919 306 SlLAANNLLAGLRG 319
1GDH_B     299 A-HQANDLIDALFG 311

 

The following are pictures of the 3-D structure of 1GDH Chain B, D-Glycerate Dehydrogenase        (Apo Form) (E.C.1.1.1.29):

Note: Tertiary structures shown.

 

** While working on this project, a new link was added to NCBI on May 1st, 2005.  Protein tag number Q9UBQ7 (328 aa), shows additional information on the GRHPR protein9.  See chart below.

Location Description
1..328 Gene
1..328 Protein
245 "active" "by similarity"
274 "active" "by similarity"
293 "active" "proton donor (By similarity)"

[CAUTION] Ref.3 sequence differs from that shown due to a frame shift in position 237.
[CAUTION] Ref.5 sequence differs from that shown due to frame shifts in positions 109 and 137.

 

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ã Eliezer Eisenberger April 2005

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