Objective -- Education -- Advanced Coursework -- Research Experience
Research Skills -- Computer Skills -- Interests & Activitees -- References

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Temple University

Bachelor of Science Degree in Biochemistry

• 3^rd/4^th year GPA total: 3.39 
• 3^rd/4^th year GPA in major: 3.33 
• Dean’s List 

9/1998 - 11/1998      Albert Einstein Medical Center Philadelphia, PA 

Emergency Medical Technician B Certification 

   + CPR, BTLS, and ER rotations

• Laser confocal microscopy, 
• Video microscopy, 
• Ratiometric imaging, and 
• Immunocytochemical staining 

Data resulting from this research was presented at the 1997
Society for Neuroscience Convention at Cold Spring Harbor.

As laboratory research technicianin in JHW's lab, involvement with
several investigators studying various aspects of neurological gene
therapy using a murine MPS-VII model (a lysosomal storage disease)
entailed the following responsibilities:

• Troubleshooting and optimization of PCR, using a mismatch primer
  for the purpose of mutant, carrier, and normal mouse genotype
  analysis
• Cell/tissue culture (maintenance of cell line, etc.)
• Animal care and maintenance of experimental mouse colony
• Small animal surgery (neurological, in utero, etc.) and
  perfusions -assisted
• Preparation and preliminary implementation of animal colony
  breeding software and corresponding computer network
• Vector selection (comparison of specificity, relative
  expression, and effectiveness of delivery) -assisted
• Interpretation of experimental protocols and data derived
• Maintenance of radiation safety, cell storage, and inventory
  logs
• Assisted lab manager in lab management, organization, and
  maintenance (solution preparation, dishwashing, autoclaving,
  ordering of biomolecules from cell center, etc.)

6/2000 – University of Pennsylvania, School of Medicine
__1/2001__Institute for Human Gene Therapy
___________________________________________________James M. Wilson, M.D.

Research Specialist B

• Nucleic acid synthesis and processing

• Nucleic acid purification (PAGE, OPC, etc.)

• Maintenance of oligonucleotide sequence and ordering database

• Nucleic acid sequencing

• Development, troubleshooting, and optimization of PCR protocols
  for the purpose of genotyping several experimental and breeding
  transgenic mouse colonies.

• Analysis and interpretation of results

• Plasmid purification -assisted

• Vector development and cloning -assisted

1/2001 - University of Pennsylvania, School of Medicine
__________ Departments of Neurosurgery and Bioengineering
______________________________________________Tracy McIntosh, Ph.D.

Experimental Biochemistry Techniques

• Gel Electrophoresis (one- and two- dimensional SDS-PAGE,
  agarose gel electrophoresis, isoelectric focusing, etc.) 
• Southern Blot, Western Blot, and Northern Blot (observed) 
• Immunohistochemical Staining Techniques 
• Spectroscopic Analysis (protein assays, nucleic acid
  assays, UV, fluorescent, IR, etc.) 
• Chromatographic Separations and Purifications
  (affinity, ion exchange, gel filtration, GC, HPLC,
  column, thin layer, paper, etc.) 
• Centrifugation Techniques (high-speed, gradient, etc.) 
• Protein Sequencing/Sequence Analysis (Edman degradation,
  Sanger method) –studied 
• Protein Measurement Techniques (Biuret and Lowry assays,
  Bradford assay, etc.) 
• ELISAs (observed)
• Scintillation Counting 

Molecular Biology Techniques

• Cloning (vector selection, DNA Purification, construction
  of chimeric DNA, etc.) –assisted 
• PCR Gene Amplification 
• In-situ Hybridization -assisted 
• Restriction Digests and Mapping, RFLP Mapping 
• Gene Isolation/Probe Techniques (Southern Blot, etc.) 
• DNA Sequencing/Sequence Analysis (Maxam-Gilbert method,
  Sanger dideoxy method, and automated sequencing with an
  attached fluorescent label) 
• Enhancer Trap, CAT assay (studied), utilization of reporter 
  genes & products (lacZ, gusB, etc.), and two-hybrid systems 

• Intel- and AMD- based PC’s 
• Apple Macintosh and Power PC 
• SGI Indigo workstation 

Operating Systems (setup, use, configuration, troubleshooting, and informal teaching/tutoring)

• Microsoft Windows (3.x, 95, 98, and NT) 
• Mac OS (through OS9)
• MS-DOS 
• Unix 
• Linux/GNU (GNOME, KDE, Red Hat, Mandrake, and Corel interfaces/distros) 

Relevant Software Applications (setup, use, configuration, troubleshooting, 
                                                              and instruction)

• Genetic analysis software such as MacVector, etc.
• Molecular modeling and mechanics computation software
  such as RasMol 
• Image acquisition and analysis software such as Cytos,
  which Dr. Paul Myers (see "research experience") wrote and
  developed, demonstrating to me the usefulness of being able
  to program software to better perform the task(s) at hand 
• Data preparation and presentation software including
  Microsoft Office (consisting of Word, Excel, Power Point,
  Publisher, and Outlook), Corel WordPerfect Suite, and
  Adobe Acrobat 
• Digital Image Enhancement and Analyses software including
  Adobe PhotoShop and Microsoft Photo Editor 
• Internet Publishing and Productivity Applications
  including Microsoft Internet Explorer, Microsoft Front
  Page, Allaire HomeSite, Netscape Communicator (consisting
  of Navigator, Messenger, Netcaster, and Composer), etc.

Programming

• HTML Editing
• JavaScript Editing
• Basic
• **Excellent typing skills and the ability to readily adapt to any
  __environment
• **Extensive internet, intranet, and BBS experience (research,
  __academic, personal, and otherwise)

In addition to my academic interests, I love to play guitar,
drums, piano, and just about any other instruments I can get 
my hands on. I also sing and enjoy writing and performing my
own original music. In order to maintain good physical as well 
as mental fitness, I like to work out, inline skate, ski/snowboard, 
swim, bike, and run.