ZAC

Pleomorphic adenoma gene-like 1 (PLAGL1) (Kas K. et al., 1998)
"Lost on transformation" (Lot1) (Abdollahi A. et al., 1997)

Gene: maps to 6q24-q25, a recognized breast cancer hot spot on chromosome 6q (Varrault A. et al., 1998; Bilanges B. et al., 1999).
mRNA: sizes: 2.6 kb and 3.2 kb.
Protein: a widely expressed zinc finger protein involved in induction of apoptosis and control of cell cycle progression.

Cell lines:
- A survey of 8 breast cancer cell (BCC) lines showed a deeply reduced (3 BCC lines) or complet loss (5 BCC lines) of ZAC expression. Treatment of 3 of these BCC lines with the methylation interfering agent 5-azacytidine induced ZAC re-expression (Bilanges B. et al., 1999).

Tumors:
- No mutation was found in ZAC coding region in a panel of 45 breast tumors with allelic imbalance of 6q24-q25. However, Northern blot and RNase protection assay analysis of ZAC expression in 23 unselected primary breast tumors showed a reduced expression in several samples (Bilanges B. et al., 1999).

Abdollahi A. et al. (1997)     Cancer Res. 57, 2029-2034.
Bilanges B. et al. (1999) Loss of expression of the candidate tumor suppressor gene ZAC in breast cancer cell lines and primary tumors. Oncogene 18, 3979-3988.
Kas K. et al. (1998) Transcriptional activation capacity of the novel PLAG family of zinc finger proteins. J. Biol. Chem. 273, 23026-23032.
Varrault A. et al. (1998) hZAC encodes a zinc finger protein with antiproliferative properties and maps to a chromosomal region frequently lost in cancer. Proc. Natl. Acad. Sci. USA 95, 8835-8840.

Under construction