Determining Annealing Temperature for Oligos

equation from DE

TMCalculation determined as follows:

y=2*(G+C) + (A+T)
TM = [22+1.46y]-1
Use the temperature of the lowest primer in your pair.

 

 

Oligo Calculator

To use this calculator, you must be using Netscape 2.0 or later, or other javascript enabled browser.
Enter Oligo Sequence in Box
Length Melting Temperature (Tm) °C
%GC content Molecular Weight: daltons (g/M)
If OD260 = 1 then have picomoles.
Original page written in Javascript by Eugen Buehler, Research Support Facilities, Department of Molecular Genetics and Biochemistry University of Pittsburgh School of Medicine.
Modified by Y. Evrard

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