Inoculate single bacterial colony in 100 µl bacterial growth medium containing appropriate antibiotic on 96-well round bottom plate

Grow at 37^oC at 800 RPM using microplate attachment for Thermomixer R ( 2hr to overnight).

Transfer 50 µl to Eppendorf (ED) conical bottom PCR plate well. Pellet in table-top centrifuge 10’ at RT (at 4000rpm).
Remove medium by aspiration.

Lyse bacterial pellet by adding 50 µl lysis buffer (see PCR screening protocol) and shaking at 1400 RPM 15’ in Thermomixer R at RT.

Continue to the regular procedures.