1. Developing a transgenic pigeonpea for drought tolerance using Mannitol-dehydrogenase gene through Agrobacterium mediated transformation.
2. DNA fingerprinting of cultivated pigeonpea and two of its wild relatives by using AFLP.
3. Isolation and characterization of a green tissue-specific promoter (rbcS) from pigeonpea.
I did my PhD work in Dr P.Ananda kumar's lab, NRCPB, IARI, New Delhi. I have registered my PhD at Ch.Ch.Singh University, Meerut, under Prof. P.C.Sharma.
In addition to my PhD work I have also worked on pigeonpea transgenic development for insect resistance, developing synthetic cry1F gene, cloning of many synthetic cry gene constructs and molecular markers in chickpea, maize and rice.
Currently I am working as Post-Doctoral fellow in Entomology Department, University of Kentucky, Lexington, USA. I am working with Dr S. R. Palli. My project work here is to refine the ecdysone based geneswitch system, testing the effects of ligands required for this geneswitch on mammalian cells and shuffling the nuclear receptors in the gene switch for high efficiency.
1. AFLP paper- Genetic Resources and Crop Evolution. (Full Text)
2. VIP gene- FEMS Microbiology Letters. (Full Text)
3. Gree tissue-specific promoter- Indian Journal of Experimental Biology. (Full Text)
4. Cotton AFLP- Journal of Plant Cell Biology and Molecular Biology.
5. Cabbage transgenic- Indian Journal of Biotechnology. (Abstract)
6. Chickpea fingerprinting- Genetica. (Full Text)
Few more in pipe-line.
My CV: S.K.Panguluri's C.V