Name:___________________

PID #:___________________

 

Quiz #3: Labs 7-9

 

 

1. We have performed many tests to observe whether certain bacteria have enzymatic activity.  Match the enzyme with its function.

 

__ catalase                                    A.  converts fibrinogen to fibrin, causing blood clots

 

__oxidase                                      B. grabs an electron from an artificial electron                               

                                                           donor,  producing a purple product

__coagulase

                                                      C. converts hydrogen peroxide to water and oxygen,  

                                                          important in protecting the bacteria from the host

                                                          immune response

 

 

2. The Kirby-Bauer technique indicates the sensitivity of bacteria to certain antibiotics.  Give two reasons why you would NOT use the antibiotic that caused the widest zone of inhibition to treat a patient.

 

 

 

 

 

 

 

 

3. Explain why people with type O blood are considered to be “universal donors,” while people with type AB blood are “universal recipients.” Remember: short answers!!!!  This can be answered in 1-2 sentences.

 

 

 

 

 

 

 

 

 

 

 

 

***more questions on the back!***

4. Define the following terms

 

Bacteriophage

 

 

Auxotroph

 

 

Conjugation

 

 

5. Label the components of a direct ELISA

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

BONUS: Why did we wait twenty minutes before plating our first conjugation sample?