Loss Salivary Anticandidal Activities in HIV/AIDS Patients
Author: A.L. LIN, C.C. WANG*, Q. SHI, D.A. JOHNSON, T.F. PATTERSON AND C-K. YEH
Departments of Dental Diagnostic Science, Community Dentistry, and medicine, UTHSCSA, San Antonio TX and GRECC, Audie L Murphy V.A. Hospital, San Antonio TX.
Presenter: Mr. Chu-Chi Wang
Third Year Dental Student, UTHSCSA
摘要：
We are studying salivary anticandidal activities in order to elucidate the pathogensis of oral candida infection in HIV(+) patients. In a pilot study, Pollock et al (1992) reported that salivary anticandidal activities for AIDS patients were decreased. In order to substantiate their findings, using microassays developed by us, anticandidal activities (blastoconidial killing and inhibition of germ tube formation) were assessed in stimulated (paraffin-chewing) whole saliva collected from 18 HIV(+) patients and 34 healthy controls. The data are expressed as the median with the 25th to 75th percentiles in parentheses. The Mann-Whitney U Test was used for analysis. The CD4 count for the HIV(+) cohort was 114 (54-298)/mm3. Salivary flow rates for HIV(+) patients [1.1 (0.8-1.4) ml/min] were lower (p<.001) than controls [1.9 (1.6-2.4)]. Salivary inhibition of Candida albicans blastoconidial viability for HIV(+) patients [86.1% (77.9%-93.6%,)] was reduced (p<.001) as compared to controls [100% (97.9%-100%)]. The ability to inhibit candidal germ tube formation was also decreased (p<.001) in HIV(+) patients [77.3% (41.1%-96.1%)] as compared to controls [100% (100%-100%)]. The HIV(+) patients were divided into two groups based on whether they were taking fluconozole at the time of saliva collection (Yes and No). No difference was found between these two groups in salivary anticandidal activities. Our results support the hypothesis that impaired salivary anticandidal activities predispose HIV(+) patients to oral candidal infection.