Protocols

Online protocol databases

Protocol online

Biowww.net

Molecular Biology Procotols

BioProtocol.com

Immunoportal.com

 

Protocols from other labs

ISH:

KC Lab protocols

Lyons Lab protocols (University of Wisconsin-Madison)

Roche application manual

Anderson Lab In Situ Hybridization Protocols

In situ protocols (Stanford Genome Evolution Center)

Non-radioactive in situ hybridization (Caltech)

Real-time PCR:

Real-time RT-PCR protocol (TIGR)

Quantitative real-time RT-PCR (Laboratory of Dr. Bart Frank, Oklahoma Medical Research Foundation)

Real-time PCR (M.Tevfik Dorak)

Troubleshooting Guide for qPCR and RTqPCR (Eurogentec)

Protocol for Real-Time RT-PCR (Xiaowei Wang, Harvard)


Microinjection:

Mary Mullins's Protocol (Marine Biological Laboratory, Woods Hole)

The microinjection workshop

A Device to Hold Zebrafish Embryos During Microinjection

Microinjection of Morpholinos (Gene Tools, LLC)


Transfection:

Transfection of Mammalian Cells using Lipofectamine (Invitrogen)

Stable Transfection (The Morimoto Lab, Northwestern University)

RW Lab protocols

Real-time RT-PCR

ISH probe synthesis

Cloning of PCR products

Automated DNA sequencing

Southern blot hybridization

Northern blot hybridization

Primer extension

Competent cell preparation

Cultivation of medaka in Hypoxia Studies

RNA extraction from medaka tissues


 
 

 

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