ENERGY AND ENZYMES

I. The Role of Energy in Living Systems

II. Using Energy from the Controlled Combustion of Food

III. How Cells Speed Up Chemical Reactions

 

I. The Role of Energy in Living Systems

v  All biological processes require energy, which living organisms must extract from their environment.  This energy is used to manufacture and transform the chemical compounds that make up living cells.  The capture and use of energy by living organisms involves thousands of chemical reactions, which together are known as metabolism.

o   Chemical reactions can be grouped into sequences called metabolic pathways.

v  The laws of thermodynamics apply to living systems.

o   First Law of Thermodynamics:

 

 

o   Second Law of Thermodynamics:

 

 

v  The flow of energy and the cycling of carbon connect living things with the environment

o   Energy comes to photosynthetic organisms (PRODUCERS) in the form of sunlight.  Photosynthesis is the process of building sugar molecules from carbon and water, utilizing energy from the sun.  The energy from the sun is stored in the chemical bonds of the sugars.  Organisms that do not undergo photosynthesis (CONSUMERS) gain energy from the chemical bonds in food molecules, such as sugars and fats.

§  Remember that plants themselves also utilize the sugars that they make by photosynthesis, primarily at night. 

§  Most organisms produce CO2 as a byproduct of the energy-harvesting process (called RESPIRATION), and this CO2 is a source of carbon for photosynthesis.  In this way, carbon atoms are continually cycled from CO2 in the atmosphere to sugars made by producers and back to CO2 by respiring producers and consumers. 

 

II. Using Energy from the Controlled Combustion of Food

v  Living systems obtain energy from food by burning organic molecules such as sugars to form CO2 and water.  What would happen if this occurred as a single reaction?

o   Wood + O2            CO2 + H2O + energy (as heat and light)

 

 

o   Bomb Calorimeters

 

 

 

v  Capturing energy from foods requires the transfer of electrons

o   Oxidation is Loss

o   Reduction is Gain

o   Redox reactions are oxidation-reduction reactions

§  Combustion Reaction: Oxidation of Methane

§  Biological Reaction: Oxidation of Methane

 

v  Metabolism is all the chemical reactions within living organisms that produce complex macromolecules, such as sugar and proteins, and which break down those macromolecules to yield smaller molecules and usable energy. 

o   Anabolic Reactions create complex molecules out of smaller compounds

o   Catabolic Reactions break down complex molecules to produce usable energy

o   These reactions tend to be intimately related.

§  Energy in a living system is transferred via the universal energy carrier, ATP.  ATP breaks down to ADP + P, releasing energy in the break down. 

v  Chemical reactions involve changing the arrangement of atoms in molecules and are governed by simple transformations of energy.  Remember the basic chemical formula:

o   A + B        C + D

o   A and B are the starting materials, or reactants

o   C and D are the end results, or products

o   A + B will not necessarily react just because they are present together.  They need to be destabilized, or activated before a chemical reaction can begin and proceed.  This jumpstart is the activation energy of the reaction.

 

v  All chemical reactions tend to proceed in the direction that will result in products with greater stability and a lower energy state.  Products whose energy state is lower than that of their reactants have less energy stored in their chemical bonds; these products are therefore in a less ordered state, as favored by the second law of thermodynamics.  This encourages chemical reactions to proceed in a particular direction (“downhill”) energetically from reactants to products.

III. How Cells Speed up Chemical Reactions

v  In cells, can be acquired by random collisions of molecules in the cytosol

v  Another source of activation energy is enzymes: a specialized class of proteins that catalyze reactions (increase the speed at which the reaction proceeds). 

o   Enzymes bind to specific reactants, called substrates and brings them together in an orientation that favors the making or breaking of bonds required to form the products.

o   Enzymes are specifically tailored to promote only one reaction.

o   Enzymes cannot make impossible reactions happen nor promote a particular reaction by changing the amount of energy associated with the reactants or products.

o   Enzyme activity can be affected by certain variables:

§  Temperature

§  pH

§  Amount of substrate in the environment

§  Amount of product in the environment

§  Amount of enzymes in the environment

v  The hydration of CO2 requires catalysis: Follow along with the reaction of CO2 with H2O to produce bicarbonate.

 

 

v  The shape of an enzyme directly determines its activity

o   Each enzyme has an active site that fits only substrates with the correct three-dimensional shape and chemical characteristics.  “Matchmaker, matchmaker…”

o   Ex: Carbonic anhydrase can bind molecules of both carbon dioxide and water to its active site, promoting the hydration reaction. 

v  Sequences of reactions have energetic advantages

o   Groups of enzymes catalize multiple steps in a sequence of chemical reactions known as a metabolic pathway

§  This enables the product of one reaction to immediately become a reactant in another reaction.

§  Metabolic pathways are how most chemical building blocks of the cell are built (ex: amino acids and nucleotides).

§  Enzymes are arranged in the cell so as to promote the multiple chemical reactions of metabolic pathways. 

 

Ø  Metabolic Rates and Lifespan

 

Ø  Metabolism and Healthy Living

 

 

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