EXPERTISE

Microbiolgy
Anaerobic fermentation; mathematical modeling of microbial growth and fermentation process using computer programs; and microbial assay of various products.

Biochemistry
Protein techniques – Ion-exchange; gel filtration; affinity and adsorption chromatography; electrophoretic techniques; one dimensional and two dimensional PAGE; isoelectric focussing; immunoelectrophoresis; western and dot blotting; electroelution of proteins and enzyme kinetics.
Chromatography – Thin layer chromatography (TLC); gas liquid chromatography (GLC); high performance liquid chromatography (HPLC).

Molecular Biology
Isolation, purification, and sequencing of DNA (using ABI 3700 automated DNA sequencer); PCR; RFLP; RAPD; AFLP; SSCP; SNP; Gene tagging and marker-assisted selection; Genescan gel (using Laser densitometer with Gelscan XL software).
Chemical and physical mutagenesis; microbial transformation.

Computers
Operating systems: Windows-NT & UNIX; Programming skills:C, C++ & Java (JSP); Databases: Oracle & MS-Access.
Internet, HTML, Web page designing, Working knowledge of statistical packages such as ‘Indostat’ and ‘SPSS’, Graphical designing, etc.

Bioinformatics
Extensively used SWISSPROT, NBRF/PIR, GenBank and GeneSeq and DNA databases; Biological research tools such as GCG software, BLAST searching; Web-based methods for identification of Microsatellites and for designing of primers; Phylogenetic softwares "PHYLIP, WINBOOT, MEGA and MAPMAKER.