Peggy's C.V.

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Name

Pei-Tzu Peggy Yang

Studying Program

MSc of Biotechnology Wageningen UR

Student number

770422980080

Birthday

Apr 22 1977

Gender

Female

Nationality

Taiwan

Education

Time

Degree

School

Department

2001 Aug~
2003 Mar

Master of Science

Wageningen UR
(Wageningen, Netherlands)

Biotechnology

1995 Sep ~
1999 Jun

Bachelor of Science

National Taiwan University
(Taipei, Taiwan)

Agronomy

Working Experience

Time

Title

Place

1999 Aug ~
2001 Jul

Research assistant

Institute of BioAgricutural Sciences, Academia Sinica
(Taipei, Taiwan)

Research Experience

Time

Laboratory

Subjects

2002 Jul~
2003 Mar

Dr. Hans de Jong, (Wageningen UR, NL)
Dr. Peter van Dijk, (NIOO, NL)

Genetic Analysis of the diplospory region of dandelion (Taraxacum officinale)

1999 Aug ~
2001 Jul

Dr. Ming-Tsair Chan, (IBAS, Academia Sinica, Taiwan)

1.  Expression of Arabidopsis transcription factor CBF1 in tomato for increasing stress resistance

2.   Tissue culture of Arabidopsis, tobacco, tomato, potato, and orchids

1999 Jul ~
1999 Aug

Dr. Ron Senderoff, (Biochem and Forestry dep. North Carolina State University, USA)

Cloning cad gene of maize

1997 Aug ~
1999 Jun

Dr. Li-Fei Liu, (Agronomy dep. National Taiwan Univeristy, Taiwan)

1.   Large scale production of human albumin with transgenic rice suspension cells

2.   Tissue culture of Rice

Publication

Heterology Expression of the Arabidopsis C-Repeat/Dehydration Response Element Binding Factor 1 Gene Confers Elevated Tolerance to Chilling and Oxidative Stresses in Transgenic Tomato, Plant Physiology, July 2002, Vol. 129, pp. 1086–1094

Techniques

Agrobacterium-mediated transformation, particle bombardment, tissue culture, cloning, PCR, northern blot analysis, southern blot analysis, western blot analysis, ELISA, isotope operation, plant physiological activity, enzyme activity analysis, FISH, library screening, etc

Important Courses & Marks in Biotechnology MSc

Subjects

Mark

Subjects

Mark

Biochemical Analysis Methods

8

Microbial genetics

8

Biochemical Separation Methods

8

Basic Elements of Genetics

9

Botanical Somatic Cell Genetics

8

Molecular Cellular Genetics

8

Cell physiology II/Biochemistry

9

Molecular Virology

9

Molecular Plant, Cell Biology

8

Biotechnology in Crop Protection

7

Plant Cell and Tissue Culture

8

Case Studies Bioprocess Engineering

8

Recombinant DNA and Genetic Manipulation

8

Practice Molecular Virology

8

MSc Thesis-
Genetic Analysis of the diplospory region in dandelion (Taraxacum officinale)

Apomixis, asexual production of seeds, is controlled by diplospory, parthenogenesis, and autonomous endosperm development. Diplospory is manipulated by Diplosporous gene (DIP). For studying the DIP gene in Taraxacum, we are going to use FISH technology in order to locate DIP on chromosomes. The approach starts from SCAR markers, which are tightly linked to DIP gene. The SCAR markers are used as probes for screening a Taraxacum genomic DNA phage library, which can provide us suitable DNA fragments (around 15 KB) for FISH technology. In addition, we will also use FISH to study the methylation on DIP region in triploid apomic plants (Ddd). We expect the methylation will be more on heterochromatin, furthermore, the DIP region in the non-apomic plants (dd), and two of the chromosomes (d/d) which do not express DIP in apomic plants (Ddd).

Reference People

Dr. Hans de Jong

[email protected]

Tel. 31 (0)317 482618; fax 31 (0)317 483146

Laboratory of Genetics, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands

Dr. P.J. van Dijk

[email protected]

Tel: 31 (0)264 791111

NIOO-Centre for Terrestrial Ecology, Boterhoeksestraat 48, 6666 GA, Heteren, The Netherlands

               

 

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