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PURPOSE
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To evaluate the effects on tumor apoptosis and
surrounding liver tissue toxicity of 3-BrPA administered intraarterially
either as a 1-hour continuous infusion or serial bolus in the rabbit Vx-2
model of liver cancer.
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METHOD AND MATERIALS
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Vx-2 tumor cells were implanted (0.1-0.2ml) in the liver
of New Zealand White rabbits. The animals were divided into treatment
groups of 4 animals each (3 study animals, 1 control) and were treated 14
days after implantation with an intraarterial injection of 3 BrPA (doses
ranging from 0.5mM to 2.5mM). After selective catheterization of the left
hepatic artery, 25 milliliters of each dose was administered either as a
1-hour continuous infusion, or two serial boluses of 12.5cc each
administered 1 hr apart. The animals were sacrificed 48 hrs after
treatment. Livers were submitted for histolopathologic analysis.
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RESULTS
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All animals treated with 3 BrPA regardless of the dose
employed showed tumor apoptosis when compared to control animal. The
animals treated with serial injections of 3 BrPA demonstrated an increase
in tumor cell death from 70% at 0.5 mM to about 90% at 2.5mM, as compared
to about 30% for the control animals. At the higher doses (2 and 2.5 mM),
there was significant liver toxicity at histopathology. The animals treated
with the 1-hour continuous infusion of 3 BrPA demonstrated an increase in
tumor cell death from 70% at 0.5 mM to about 100% at 1.75mM. However, in
contrast to the other group, there was no liver toxicity associated with this
treatment.
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CONCLUSION
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Intraarterial injection of 3 BR-PA resulted in tumor
cell death in all treated animals. The 1-hour intraarterial infusion of
3-BrPA resulted in complete tumor destruction and was significantly greater
than that of serial bolus injection. In addition, animals treated in this
manner had no liver toxicity.
|
|
|
|
|
|
PURPOSE
|
|
|
To evaluate the effects on tumor apoptosis and
surrounding liver tissue toxicity of 3-BrPA administered intraarterially
either as a 1-hour continuous infusion or serial bolus in the rabbit Vx-2
model of liver cancer.
|
|
|
|
|
|
METHOD AND MATERIALS
|
|
|
Vx-2 tumor cells were implanted (0.1-0.2ml) in the liver
of New Zealand White rabbits. The animals were divided into treatment
groups of 4 animals each (3 study animals, 1 control) and were treated 14
days after implantation with an intraarterial injection of 3 BrPA (doses
ranging from 0.5mM to 2.5mM). After selective catheterization of the left
hepatic artery, 25 milliliters of each dose was administered either as a
1-hour continuous infusion, or two serial boluses of 12.5cc each
administered 1 hr apart. The animals were sacrificed 48 hrs after
treatment. Livers were submitted for histolopathologic analysis.
|
|
|
|
|
|
RESULTS
|
|
|
All animals treated with 3 BrPA regardless of the dose
employed showed tumor apoptosis when compared to control animal. The
animals treated with serial injections of 3 BrPA demonstrated an increase
in tumor cell death from 70% at 0.5 mM to about 90% at 2.5mM, as compared
to about 30% for the control animals. At the higher doses (2 and 2.5 mM),
there was significant liver toxicity at histopathology. The animals treated
with the 1-hour continuous infusion of 3 BrPA demonstrated an increase in
tumor cell death from 70% at 0.5 mM to about 100% at 1.75mM. However, in
contrast to the other group, there was no liver toxicity associated with this
treatment.
|
|
|
|
|
|
CONCLUSION
|
|
|
Intraarterial injection of 3 BR-PA resulted in tumor
cell death in all treated animals. The 1-hour intraarterial infusion of
3-BrPA resulted in complete tumor destruction and was significantly greater
than that of serial bolus injection. In addition, animals treated in this
manner had no liver toxicity.
|
|
|
|
- Disclosure
information unavailable 