Professional scientist with wide expertise in immunochemistry, protein and nucleic acid chemistry, and enzymology. Researcher with extensive knowledge of quantitative assays, design of experiments and statistical analysis, broad experience with sequence databases, and decent programming skills. Expert in commercial DNA sequencing, analysis of DNA sequencing data, and genome sequencing projects.

    Protein engineering. Development of new products and processes for gene technologies (Introduction and characterization of thermostable DNA polymerases, chimeric DNA polymerases, proteins for DNA amplification and modification).

    Development of DNA sequencing and PCR applications.

    Development and characterization of metal-specific monoclonal antibodies.

    Development of quantitative immunoassays for heavy metals in environmental samples (ELISA).

    Thechnical experience:

    Industrial DNA sequencing and sequence analysis; use of ABI Prism DNA sequencers; PCR.
    Industrial design of primers for DNA sequencing and PCR.
    Protein purification (LC, FPLC, HPLC).
    Electrophoresis of proteins and nucleic acids under native and denaturing conditions (PAG and agarose gels).
    Gel mobility shift assays. Blotting.
    Electrophoretic purification of DNA and RNA. Capillary electrophoresis.
    Quantitative analysis of proteins and nucleic acids in gels.
    Plasmid DNA purification from transformed cells. Purification of genomic DNA.
    In vitro DNA transcription. Studies of protein - RNA and protein - DNA interactions.
    Research on kinetics of enzymatic reactions.
    Use of spectrophotometric and fluorometric methods for analysis of proteins and nucleic acids.
    Chemical modification of proteins including synthesis and characterization of low molecular weight haptens conjugates
    with carrier proteins for immunization and characterization of antibodies.
    Use of Kinetic Exclusion Assay (KinExA) to study equilibrium and kinetic parameters of interactions between antibodies
    and antigens in solutions.
    Use of spectrophotometric and EPR methods for characterization of metal complexes.
    Pulse radiolysis, analysis of kinetics of short time free - radicals processes. Characterization of interactions of free radicals
    with antioxidants.
    Protein, DNA and RNA sequence and structure analysis. Work with sequence and structural data banks, secondary
    structure prediction. Computer modeling of chemical structures (protein domains, DNA, RNA, complexes of metals with
    chelates). Use of energy minimization and molecular dynamics methods in modeling.

    COMPUTER HARDWARE: PC, Macintosh, Iris-Indigo (SiliconGraphic), Sun

    SOFTWARE: DOS, WINDOWS and applications, Unix, Linux, FORTRAN, BASIC, InsightII (Biosym Tech),
    Discover (Biosym Tech), phred, phrap, crossmatch, consed.

    Development of new procedures that improve PCR and DNA cycle sequencing with difficult targets. Study of DNA-processing enzymes from thermophiles.

    Development of monoclonal antibodies and immunochemical procedures for analysis of heavy metal contaminations in the environment; study of antibody structure/function relationships; development, theoretical justification, and experimental validation of new systems for immunoanalysis; design of immunosensors for environmental analysis.

    Allosteric regulation of enzymes, enzyme kinetics of oxidases, reductases, phosphohydrolases, DNA polymerases; mechanisms of specificity of protein-protein, protein-DNA and protein-RNA recognition; study of structural and functional features of DNA replication systems; structural principles of recognition of nucleic acid substrates, regulators, and accessory proteins by DNA polymerases; role of RNA - protein interactions in formation and operation of DNA replication complexes; dynamic modeling of interactions of multienzyme systems with nucleic acids.

    Biochemistry of visual function, lens metabolism; theoretical description of intact biochemical systems. Reactions of free radicals in biological systems; kinetics of free radicals reactions; application of pulse radiolysis for studies of biological systems.

    2000 - present: Senior Scientist, Fidelity Systems, Inc., Gaithersburg, MD, USA.

    1996 - 2000: Postdoctoral Research Associate, Department of Ophthalmology, Tulane University School of Medicine, New Orleans, LA, USA with Dr. Diane A. Blake.

    1994 - 1995: Research Assistant Professor, Department of Biochemistry, Tulane University, New Orleans, LA, USA with Dr. Jim D. Karam.

    1991 - 1994: Postdoctoral Fellow, Department of Biochemistry, Tulane University, New Orleans, LA, USA with Dr. Jim D. Karam.

    1991: Research Scientist, Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA with Dr. Jim D. Karam.

    1989 - 1992: Research Scientist, Laboratory of Kinetics of Biochemical Processes, A.N.Bach Institute of Biochemistry, Academy of Sciences, Moscow, USSR; Head of the Research Group Oxidation in Cataractogenesis.

    1984 - 1988: Junior Research Scientist, Laboratory of Kinetics of Biochemical Processes, A.N.Bach Institute of Biochemistry, Academy of Sciences, Moscow, USSR with Dr. Ilya V. Berezin; since 1986 - with Dr. Alexander I. Yaropolov.

    Ph.D. in Chemistry, 1986, M.V. Lomonosov Moscow State University, Moscow, Russia (A.N.Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry and School of Chemistry).

    Thesis directors: Dr. Svetlana M. Avaeva and Dr. Alexander A. Baykov

    Thesis title: "Regulation of Inorganic Pyrophosphatase Activity by Substrates."

    M.S. in Chemistry, 1981, M.V. Lomonosov Moscow State University, Moscow, Russia (School of Chemistry).

    Fluent: English, Russian

    Reading: French

    Lecturing to medical residents of the Department of Ophthalmology at Tulane University on biochemistry of visual function (1996).

    Peer-reviewing for J. Nephrology (1996).

    American Chemical Society

    Blake, R.C.II, Pavlov, A.R., Khosraviani, M., Ensley H.E., Kiefer G.E.,Yu, H., Li X., and Blake, D.A. Novel monoclonal antibodies with specificity for chelated uranium(VI): isolation and binding properties. Bioconjug Chem., Vol. 15, p. 1125-1136, 2004.

    Pavlov, A.R., Pavlova, N.V., Kozyavkin, S.A., and Slesarev A.I. Recent developments in the optimization of thermostable DNA polymerases for efficient applications. Trends Biotechnol., Vol. 22, p.253-260, 2004.

    Pavlov, A.R., Belova, G.I., Kozyavkin, S.A., and Slesarev A.I. Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases. Proc. Natl. Acad. Sci. USA, Vol. 99, p.13510-13515, 2002.

    Blake, D.A., Blake, R.C.II, Pavlov, A.R., Jones R.M., Darwish, I.A.M., and Yu, H. Antibody-based sensors for heavy metals. Biosens. Bioelectronics., Vol. 16, p. 799-809, 2001.

    Pavlov, A.R. and Karam J.D. Nucleotide-sequence-specific and nonspecific interactions of T4 DNA polymerase with its own RNA. Nucleic Acids Res., Vol. 28, p.4657-4664, 2000.

    Khosraviani, M., Blake, R.C. II, Pavlov, A.R., Lorbach, S.C., Yu, H., Delehanty, J. B., Brechbiel, M.W., and Blake, D.A. Binding properties of a monoclonal antibody directed toward lead-chelate complexes. Bioconjugate Chem., Vol. 11, p.267-277, 2000.

    Blake, R.C.II, Pavlov, A.R., and Blake, D.A. Automated kinetic exclusion assays to quantify protein binding interactions in homogeneous solution. Analytical Biochemistry Vol. 272, p.123-134, 1999.

    Blake, D.A., Blake, R.C.II, Khosraviani, M., and Pavlov, A.R. Immunoassays for metal ions. Analytica Chimica Acta, Vol. 376 p.13-19, 1998.

    Wang, C.-C., Pavlov, A.R., and Karam J.D. Evolution of RNA-binding specificity in T4 DNA polymerase. J. Biol. Chem. Vol. 272, No 28, pp. 17703-17710, 1997.

    Pavlov, A.R.and Karam, J.D. Binding specificity of T4 DNA polymerase to RNA. J. Biol. Chem. Vol. 269, No 17, pp. 12968-12972, 1994.

    Sergeev, A.G., Pavlov, A.R., Revina, A.A., and Yaropolov, A.I. The mechanism of interaction of ceruloplasmin with superoxide radicals. Int. J. Biochem., Vol.25, No. 11, pp. 1549 1554, 1993.

    Pavlov, A.R., Revina, A.A., Dupin, A.M., Boldyrev, A.A., and Yaropolov, A.I. The mechanism of interaction of carnosine with superoxide radicals in water solutions. Biochim. Biophys. Acta, Vol. 115, No.7, pp. 304 312, 1993.

    Vartanov, S.S., Pavlov, A.R., and Yaropolov, A.I. Aldose reductase physiological role, properties and regulation of its activity. Biochemistry (Russsia) Vol.57, No.3, pp.205 218, 1992.

    Pavlov, A.R., Vartanov, S.S., and Yaropolov, A.I. Regulation of lens aldose reductase activity. Kinetic mechanism for activation of the enzyme. Biochemistry (Russsia) Vol.57, No.3, pp.247 254, 1992.

    Pavlov, A.R., Vartanov, S.S., and Yaropolov, A.I. Regulation of lens aldose reductase activity. Influence of effectors on reversible isomerization of the enzyme. Biochemistry (Russsia) Vol.56, No.11, pp.1412 1423, 1991.

    Vartanov, S.S., Pavlov, A.R., and Yaropolov, A.I. Regulation of lens aldose reductase activity. Non- hyperbolic kinetics of NADPH oxidation by glucose. Biochemistry (Russsia) Vol.55, No.11, pp. 2046 2057, 1990.

    Pavlov, A.R., Revina, A.A., Doupin, A.M., Boldyrev, A.A., and Yaropolov A.I. Interaction of carnosine with superoxide radicals in aqueous solutions. Bulletin of experimental biology and medicine (Russsia) Vol.108, No.10, 1990 pp.526 529.

    Baykov, A.A., Shestakov, A.S., Pavlov, A.R., Smirnova, I.N., Larionov, V.N., and Avaeva, S.M. The interaction of phosphate and manganese ions with baker's yeast inorganic pyrophosphatase. Biochemistry (Russsia Vol.54, No.6, pp. 796 803, 1989.

    Baykov, A.A., Pavlov, A.R., Kasho, V.N., and Avaeva, S.M. Allosteric Regulation of Yeast Inorganic Pyrophosphatase by Substrate. Archives of Biochemistry and Biophysics. Vol. 273, No.2, pp.301 309, 1989.

    Sergeev, A.G., Pavlov, A.R., Zhazhina, E.O., Basevich, V.V., and Yaropolov, A.I. Influence of ceruloplasmin, laccase and superoxide dismutase on the level of the superoxide radicals. Biopolimers and cells (Russsia), Vol.4, No.5, pp. 245 250, 1988.

    Pavlov, A.R., Baykov, A.A., and Avaeva, S.M. The regulatory site of inorganic pyrophosphatase. The role of activating in its manifestation. Biochemistry (Russsia) Vol.51, No.3, pp. 369 387, 1986.

    Baykov, A.A., Pavlov, A.R., and Avaeva, S.M. The regulatory site of inorganic pyrophosphatase. Interaction with substrate analogs. Biochemistry (Russsia) Vol.51, No.2, pp. 202 211, 1986.

    Pavlov, A.R., Baykov, A.A., and Avaeva, S.M. The regulatory site of inorganic pyrophosphatase. Non hyperbolic kinetics of the enzymatic reaction at low substrate concentrations. Biochemistry (Russsia) Vol.51, No.2, pp. 179 187, 1986.

    Pavlov, A.R., Baykov, A.A., and Avaeva, S.M. Studies of pyrophosphate synthesis in the active site of inorganic pyrophosphatase. Vestnik Moskovskogo Universiteta. Khimiya (Russsia) Vol.26, No.6, pp. 608 611, 1985.

    Volk, S.E., Pavlov, A.R., and Baykov, A.A. Membrane inorganic pyrophosphatase. The role of SH group in hydrolytic activity. Bioorganic chemistry (Russsia) Vol. 10, No.2, pp. 204 212, 1984.

    Pavlov, A.R., Pavlova, N.V., Kozyavkin, S.A., and Slesarev A.I. Thermostable Chimeric DNA Polymerases with High Resistance to Inhibitors In: DNA Amplification: Current Technologies and Applications. (Demidov, V.V and Broude, N.E. eds.) Horizon Bioscience, Norwich, UK, pp. 3-20, 2004.

    Blake, D.A., Khosraviani, M., Pavlov, A.R., Blake, R.C. II. Characterization of a metal-specific monoclonal antibody. In: Immunochemical Technology for Environmental Applications. (Aga, D.S. and Thurman, E.M. eds.) American Chemical Society Series, Vol. 657, American Chemical Society, Washington, DC, USA, pp. 49-60, 1997.

    Blake, D.A., Pavlov, A.R., Khosraviani, M., Blake, R.C. II. Determination of antibody affinity and specificity. In: Proc. Immunochemistry Summit V and Joint National Conference for Environmental Health Monitoring. (Joseph, J.M. and Bober, A. eds.) Association of State and Territorial Public Health Laboratory Directors, Washington, DC., USA, pp. 95-106, 1997.

    Blake, D.A., Pavlov, A.R., Khosraviani, M., Flowers, G.C. Immunoassay for cadmium in ambient water samples. In: Current Protocols in Field Analytical Chemistry, (Lopez-Avila, V. ed.), John Wiley and Sons, Inc., New York, USA, pp. 3C.1.1-3C.1.10, 1998.

    Pavlov, A.R., Khosraviani, M., Chaiban, J.E., Blake, D.A. Strategies and problems in the isolation of metal-specific monoclonal antibodies. In: Antibody Engineering II: New Technology, Application and Commercialization. Vol II, IBC USA Publishing, Southborough, MA, pp. 5.1.1-5.1.20, 1997.

    Blake, D.A., Blake, R.C.II, Pavlov, A.R., Ensley H., and Yu, H. Antibody - based sensors for heavy metal ions. #B00310, Six World Congress on Biosensors, San Diego, May 24-26, 2000.

    Pavlov, A.R., Blake, D.A., and Flowers, G.C. Determination of lead in environmental water samples by a rapid and portable immunoassay. #33, 219th ACS National Meeting, San Francisco, March 26-30, 2000.

    Blake, D.A., Pavlov, A.R., Khosraviani, M.,Yu, H., Ensley H., and Blake, R.C.II. Antibodies and antibody-based immunobiosensors for hexavalent uranium. #202 219th ACS National Meeting, San Francisco, March 26-30, 2000.

    Khosraviani, M., Pavlov, A.R., Blake, R.C.II, and Blake, D.A. Isolation and characterization of a Pb(II)-specific monoclonal antibody. 214 National ACS Meeting and Immunochemistry Summit VI, Las Vegas, September 7-10, 1997.

    Blake, D.A., Khosraviani, M., Pavlov, A.R., and Blake, R.C. II. Generation and properties of metal-specific monoclonal antibodies. Abst. #93, International Biometals Symposium. Calgary, Canada. August 10-14, 1997.

    Blake, R.C., Pavlov, A.R., Khosraviani, M., and Blake, D.A. Binding specificity of an anti-metal antibody. ASBMB/ASIP/AAI Joint Meeting. June 1-6, New Orleans. Abst #LB39, p. 22., 1996.

    Wang, C.-C., Pavlov, A.R., and Karam, J.D. Mapping the RNA specificity determinants of T4 DNA polymerase. Eleventh Evergreen International T4 Meeting. Abst #9. Olympia, Washington, August 8-12, 1996.

    Wang, C.-C., Pavlov, A.R., and Karam, J.D. Determinants of T4 DNA polymerase function. FASEB J. Vol. 10, No 6, p. A1234, 1996.

    Ghindilis, A.L., Kiselev, B.S., Mikaelyan, A.L., Pavlov, A.R., Salakhutdinov, V.K., and Yaropolov, A.I. Optical memory on the biochemical structure. Proceedings IIIth All Union Conference "Problems of optical memory", Erevan, pp.127 128, 1987.

    etc…

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