microscopes it is an instrument for viewing very small objects. A simple microscope is a convex lens of short focal length. A compound microscope gives a larger magnification with less aberrations. It has an objective of short focal length and an eye piece * . The objective forms a real inverted and magnified intermediate image. This image is further magnified by the eye piece. The final magnification, M is the product of linear magnification produced by the objective, M0 and the angular magnification of the eyepiece, Me.
M = M0.Me
Generally M0 and Me are separately labeled.
The numerical aperture, NA of microscope given by,
NA = m sin a
where m is the refractive index of the medium between the object and the objective and a is half angle of the cone of light entering objective. The numerical aperture is increased with oil immersion objectives (see fig. m10).