By using Peptide mass fingerprint analysis of 6 purified proteins which had been added with DTT, iodoacetamide, and digested with Trypsin, We found their mass as following in this link.

Then find the names of 6 proteins and their accession numbers after that calculate their pIs and molecular weights.

 

The results show in this table

Sample No. Proteins name Acession No. Source Theoretical pI/Mw (monoisotopic)
1

Betaine-aldehyde dehydrogenase (BADH).

O24174
Oryza sativa
6.63 / 54613.12
2

ALDH10A9; aldehyde dehydrogenase/ oxidoreductase [Arabidopsis thaliana].

NP_190400
Arabidopsis thaliana (thale cress)
5.39 / 54883.06
3

neuramindase [Influenza A virus (A/Viet Nam/3062/2004(H5N1))].

AAT73330
Influenza A virus (A/Viet Nam/3062/2004(H5N1)
6.13 / 49051.53
4
M1 protein [Influenza A virus (A/Duck/Hong Kong/646.3/01 (H5N1))].
AAO52908
Influenza A virus (A/Duck/Hong Kong/646.3/01 (H5N1)
9.45 / 27808.26
5

Somatotropin precursor (Growth hormone).

Q9W6R8
Heteropneustes fossilis (stinging catfish)
5.97 / 22523.44
6

insulin

AAA59179
Homo sapiens (human)
5.57 / 11616.85

 

 

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