How to use bioinformatics tool (NetPhos 2.0) step by step ![]() |
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NetPhos 2.0 Server this programe use to predict the phosphorylation site in eukaryote protein . Protein phosphorylation is the important regulatory event. Many enzymes and receptors are switched "on" or "off" by phosphorylation and dephosphorylation. |
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| After selected you will go into NetPhos2.0 Server.Then you can select your interested sequences that you want to know phosphorylation site in protein and paste into the input field.
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IF you do not have sequence in your local disk you can search information for your interesed sequence in internet such as by NCBI.
The following will show the way to find the interested sequence in NCBI website. |
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After that you will see NCBI webpage. You can the database type and key the name of your interested thing. They will show all data that relate to your search then your select your most interested |
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The details will be shown in GenBank format but you need FASTA format for NetPhos so select FASTA at Display. |
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After you get your interested sequence in FASTA format go back to NetPhos and paste into the input field. |
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If you waiting,the result page will up date automatically and show in the following. |
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The result will show the number and position of phosphorylation in your interested sequence and they also have the link for explaination in the bottom. |
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This page will give explanation of output format. |
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