SBIOAC BIOLOGY                                                   CELLULAR RESPIRATION

 

Name:                                          .                                          2003 02 13

 

Read questions carefully. Value follows question in parentheses. Answer all questions, as no marks will be deducted for incorrect responses. There is only ONE correct answer for each multiple choice.  Each question is worth one mark unless otherwise noted.

TOTAL =  53 + 3 bonus

 

 

1. In diffusion,     

 

a.        molecules move faster in a liquid than in a gas

b.      molecules move from a region of higher concentration to an area of lower concentration

c.       molecules move from a region of lower concentration to an area of higher concentration

d.      molecules move faster because energy is added to the system

2. If the volume of a cell increases when it is placed in an aqueous solution, that solution is said to be __________ to the cell.

                   

a. hypotonic

b. isotonic

c. hypertonic

d. gin and tonic

3. Some liver cells ingest bacteria, a function probably accomplished by . . .

a. phagocytosis.

b. pinocytosis.

c. exocytosis.

d. passive transport.     

4. The concentration of potassium in a red blood cell is much greater than in the surrounding plasma, but potassium continues to move into the cell. This is an example of                   .

 

a.       osmosis

b.      diffusion

c.       facilitated diffusion

d.      active transport

 

5. Active transport . . .

                   

a. moves substances down their concentration gradient.

 

b. moves small uncharged molecules through the lipid bilayer.

 

c. requires ATP energy.

 

d. is important in facilitated diffusion.

 

6. The sodium-potassium pump moves

            a.   Na + and  K+ into the cell

            b.   Na + and  K+ out of the cell

            c.   Na + into the cell and  K+ out of the cell

            d.   Na + out of the cell and  K+ into the cell

 

7. Enzymes can . . .

                   

a. decrease the initial energy requirement of reactions.

b. make endergonic reactions exergonic.

c. change the equilibrium point of a reaction.

d. change the net energy yield of a reaction.

 

8. Which of the following would bind to the active site of an enzyme?

 

a.             allosteric inhibitor

b.            allosteric activator

c.             competitive inhibitor

d.            non-competitive inhibitor

 

 

9. A cuboidal (cube-shaped) cell fits across a medium-power field of view 15 times.  The diameter of the field of view at this power is 1.8 mm.  A drawing of this cell (in proportion) measures 27 mm per edge.  Give the proper units with your answer. (2)

 

a.  What is the actual size of the cell? (1.8mm /15) = 0.12 mm or 120 micrometers

 

b.  What is the magnification of the drawing? drawing size/actual size = 27mm/0.12 mm = 225X  (NO UNITS!)

 

10. State clearly and completely the First and Second Laws of Thermodynamics .  (2)

 

The first law states that energy can be neither created nor destroyed, only transferred or transformed. The second law states that with every transfer or transformation of energy the entropy (disorder/randomness) of the universe increases.

 

 

11. In an organism, free energy

 

a. can be obtained at no cost to the system.

b. can be spent with no cost to the universe.

c. is equivalent to the system’s total energy.

d. is available to do work.

 

12. Energy of activation can be defined as the         .

 

a.             conversion of energy in a system

b.            the amount of randomness in a system

c.             the input of energy needed to start a reaction

d.            the free energy change in a system

13. A reaction is designated as exergonic rather than endergonic when ___________.

a. no kinetic energy is released

b. the energy of the products is less than the energy of the reactants

c. activation energy exceeds net energy release

d. activation energy is necessary

 

14. To __________ a compound means to __________; in biology, this usually results in a compound that has __________ bond energy than before the process began.

                   

a. reduce ... add electrons to it ... more

b. oxidize ... add electrons to it ... less

c. reduce ... add electrons to it ... less

d. oxidize ... take electrons from it ... more

 

15. Endergonic reactions, if left to themselves, will attain equilibrium with almost none of the reactants converted to products. But cells can make endergonic       reactions go almost to completion by ____________.

 

a. rapidly taking up their excess electrons

b. coupling them to the hydrolysis of ATP

c. coupling them to the formation of ATP

d. using allosteric enzymes to speed them up

 

16. An electron carrier acts as an energy storage molecule when it is __________ (for example, __________).

 

a. oxidized ... NADH

b. reduced ... NAD+

c. reduced ... NADH

d. oxidized ... NAD+

           

17. When glucose is completely broken down to carbon dioxide and water during aerobic respiration, more than 50 percent of its energy is released as         .

 

a.             ATP

b.            ADP

c.             carbon dioxide

            d.      heat

 

18. Of the metabolic pathways listed below, the only pathway found in all organisms is   .

 

            a. fermentation.

            b. electron transport chain.

            c. glycolysis.

            d. cellular respiration.

 

19. The primary function of fermentation is to        .

 

a.             regenerate NAD+

b.            provide alcohol to students

c.             produce NADH

d.            oxidize pyruvate

20. ATP is important because it . . . .

a.      carries the genetic information.

b.     contributes entropy to the cell.

c.     is one of the building blocks of DNA.

d.     carries energy from one chemical reaction to another.

e.     both c and d  (ATP is a ribonucleotide not a deoxyribonucleotide)

21. Substrate-level phosphorylation requires       .

 

a.             an enzyme, a phosphorylated intermediate and ADP

b.            an energy source and protons

c.             an enzyme, ADP and phosphate

d.            oxygen, electron transfer chain and protons

22. Which statement best describes oxidative phosphorylation?

 

a.       H+ ions diffuse through the inner membrane to make ATP

b.      H+ ions are actively transported out of mitochondrion to make ATP in cytoplasm

c.       H+ ions accumulate in the matrix and are used to make ATP in the Krebs cycle

d.      ATP is made in the matrix when H+ ions diffuse through a membrane enzyme

( and that membrane enzyme is ATP synthase)

23. Energy released by the breakdown of ATP can be used for                            

a. protein synthesis

b. muscle contraction

c. active transport of molecules across membranes

d. all of the above

 

24. When pyruvate is converted to acetyl CoA ___________.

                   

a. CO2 and Coenzyme A are formed

 

b. CO2 and NADH are formed

 

c. CO2 and ATP are formed

 

d. NAD+ is regenerated

 

25. OMIT THIS QUESTION

 

26. The Krebs cycle (citric acid cycle) takes place in the          .

 

 a.     cytoplasm.

 b.     nucleus.

 c.     mitochondrion.

 d.     Golgi complex.

 

27. At the end of the Krebs cycle, glucose is completely broken down into          .

 

a.      oxygen and carbon dioxide.

b.      oxygen and water.

c.             carbon dioxide and water.

d.            oxygen, carbon dioxide, and water.

 

28. Muscle tissues make lactate from pyruvate in order to . . .

                   

a. enable you to get drunk.

b. utilize the energy in pyruvate.

c. regenerate (oxidized) NAD.

d. get rid of toxic pyruvate.

29. Suppose a molecule of glucose is fermented by a cell. One of the end products is  CH3CH2OH. Without even knowing the name of this product, how do you know that energy could still be extracted from it?

                   

a. It is phosphorylated and thus energy rich.

b. The product contains C-H bonds from which energy can be harvested.

c. The product can be converted back to glucose.

d. It is water-soluble.

 

30. If we did not breathe in O2, we would not . . .

                   

a. be affected because we can switch to alcoholic fermentation.

 

b. make enough ATP to sustain life.

 

c. have enough enzymes to catalyze reactions.

 

d. be able to perform lactate fermentation.

 

31. During respiration in a eukaryotic cell, reactions of the Krebs cycle occur or are located in or on ___________.

                   

a. across the inner membrane of the mitochondrion

 

b. the cristae of the mitochondrion

 

c. the cytosol

 

d. the matrix of the mitochondrion

 

32. Which part of the cellular catabolism of glucose requires molecular oxygen (O2)?

                   

            a. Fermentation

            b. Glycolysis

            c. Krebs cycle

            d. The electron transport chain

33. In respiration, which one of the following does the electron transport chain (or its components) NOT do directly?

                   

a. Carry electrons

b. Phosphorylate ADP to make ATP

c. Regenerate (oxidized) FAD

d. Form a proton gradient

34. Cytoplasmic NAD+ is recycled in aerobic respiration at which stage?

             

a.      the conversion of pyruvate to Acetyl CoA

b       the splitting of the 6-carbon sugar into two molecules of 3-carbon sugar

c.      the reduction of pyruvate

d.      the transfer of electrons from cytoplasmic NADH through a shuttle to the mitochondrial electron transport chain.

35. The final electron acceptor of the electron transport chain is        .

 

a.             oxygen

b.            water                                

c.             NAD+

d.      glucose

36. During oxidative phosphorylation in eukaryotic cells, ATP-synthase catalyzes the

phosphorylation of ADP to form ATP. The energy needed for this endergonic reaction comes from . . .

 

a. the movement of hydrogen ions from the  intermembrane space to the mitochondrial matrix.

b. the reduction of NAD+ to form NADH.

c. the movement of FADH from the cytoplasm to the  mitochondria.

d. the fermentation of pyruvate to form lactic acid.

37. The efficiency of aerobic respiration is approximately . . .

a. 10%

b. 20%

c. 2%

d. 40%

 

38. How many ATPs are produced by substrate-level phosphorylation from the complete breakdown of a single molecule of glucose in the presence of oxygen?

                   

            a. 6 ATP

            b. 2 ATP

            c. 36 ATP

            d. 4 ATP (2 from glycolysis, 2 from the Krebs cycle)

 

39. A waste product of aerobic respiration and of alcoholic fermentation is . . .

a. carbon dioxide (CO2).

b. NAD+

c. ATP

d. oxygen gas (O2).

 

40. The major energy accomplishment of the Krebs cycle is ___________.

a. formation of NADH and FADH2

b. formation of ATP

c. formation of CO2

d. completion of substrate phosphorylation

 

41. Write the balanced CHEMICAL equation for aerobic respiration (2).

C6H12O6 + 6O2 ® 6CO2 + 6H2O (+ energy)

 

42. Showing each stage in aerobic respiration, account for each ATP generated from the complete oxidation of glucose.  Also, indicate which type of phosphorylation (do not abbreviate unless you give a legend) has occurred and in which organelle or location within the cell the process has occurred.  Assume the maximum number of ATP molecules per glucose is 36. (5)

 

SLP = substrate-level phosphorylation

OP = oxidative phosphorylation

 

Cytoplasm (glycolysis)                       2 ATP (SLP)

 

                                    2 NADH         4  ATP (OP)

 

Mitochondrion  (conversion to Acetyl CoA)

   

                                    2 NADH         6 ATP (OP)

 

                        (Krebs cycle)

                                                            2 ATP (SLP)

            

                                    6 NADH         18 ATP (OP)

 

                                    2FADH2           4 ATP (OP)

Total = 36 ATP

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