Sample Input:

1) Use your protein aa sequence of choice from a target organism

>gi|85190|pir||A29650 wingless (wg) protein precursor - fruit fly (Drosophila melanogaster) MDISYIFVICLMALCSGGSSLSQVEGKQKSGRGRGSMWWGIAKVGEPNNITPIMYMDPAIHS
TLRRKQRRLVRDNPGVLGALVKGANLAISECQHQFRNRRWNCSTRNFSRGKNLFGKIVDRG
CRETSFIYAITSAAVTHSIARACSEGTIESCTCDYSHQSRSPQANHQAGSVAGVRDWEWGGC
SDNIGFGFKFSREFVDTGERGRNLREKMNLHNNEAGRAHVQAEMRQECKCHGMSGSCTVKT
CWMRLANFRVIGDNLKARFDGATRVQVTNSLRATNALAPVSPNAAGSNSVGSNGLIIPQSGL
VYGEEEERMLNDHMPDILLENSHPISKIHHPNMPSPNSLPQAGQRGGRNGRRQGRKHNRYH
FQLNPHNPEHKPPGSKDLVYLEPSPSFCEKNLRQGILGTHGRQCNETSLGVDGCGLMCCGRG
YRRDEVVVVERCACTFHWCCEVKCKLCRTKKVIYTCL
(fasta format: start with “>”)

2) Choose 4 organisms to run BLAST against

• Mouse: 77143 sequences
• C. elegans: 62256 sequences
• Zebrafish: 3069 sequences
• Xenopus: 5174 sequences
  

Sample Output:

1) Distance Matrix from Metric Calculations

2) Phylogenetic Tree