ECL detection Kit Recipe:
Reagents:
p-coumaric acid (Sigma #9008)
3-aminophtalhydrazide (Luminol, Sigma #09253)
1 M Tris pH 8.5
30 % H2O2
Stocks:
90mM p-coumaric acid in DMSO (store at -20°C)
250 mM Luminol in DMSO (store at -20°C)
Fresh solutions:
2-3 weeks at 4°C
Solution A:
5 ml 1M Tris pH 8.5
45 ml water
110 ml 90 mM coumaric acid
250 ml 250 mM Luminol
Solution B:
100 ml 30% H2O2
900 ml water
Before usage, mix:
1 ml Solution A + 3 ml Solution B