Protocol:
add to a 1ml cuvette:
800 ml PBS
200 ml Bradford
Reagent
mix, set absorption at 595 nm to zero
add to a 1ml cuvette:
800 ml - sample volume ml PBS
1-100ml
Sample
200 ml
Bradford Reagent
mix measure absorption at 595 nm
Defining m & c values:
Measure OD595 for 1,2,5,10,20 mg
BSA
Make a plot mg BSA on the x-axis and OD595
on the y axis
Make a linear fit, m is the slope and c the crosspoint with the y axis
at 0 mg BSA
Bradford Reagent:
Biorad (store at 4�C)
PBS:
0.9% NaCl
0.1 M NaPhosphate buffer pH 7