Introduction:
Protease is protein that is hydrolyzed (or sniped) by collective enzymes. There are many types of protease, each hydrolyzing in its own unique way. Some protease cut at the end of a protein chain and cut off one amino acid at a time (exopeptides). Other proteases cut from inside out (endopeptides). Some protease is extremely specific others aren’t. 
In this lab we are using gelatin (JELLO) and powdered bacterial protease to see how protease breaks down proteins by seeing how much JELLO gels with protease in it. 
Our research questions are: what applications are there for bacterial protease, and how do you use bacterial protease. All of the testing is done in room temperature.
Hypothesis:
If there is protein in gelatin then protease will break the bonds and therefore, the gelatin will stay liquefied.
Procedures:
1.	Obtain 4 test tubes and one small package of cherry flavored, JELLO brand gelatin
2.	Boil water and follow cooking directions on product box to make gelatin
3.	Measure out 5ml of JELLO into each of the four test tubes
4.	Obtain 7 grams of powdered bacterial protease 
5.	Measure out one gram in the first test tube, two grams in the second and four grams of protease in the third test tube, but do not put any bacterial protease in the fourth test tube, that will be your control.
6.	Vigorously, but carefully, shake the test tubes to mix materials for about 5 minutes each test tube
7.	Let sit over night, observe in morning
Data/results:
Observation on day 10-23-2003
Test tube one was completely mixed and liquefied, test tube two has a little but of resin on the bottom of test tube, also liquefied test tube three had chunks of protease still clumped on the top and had soaked up some of the gelatin like a sponge, but their was liquid still on the bottom. The control had solidified gelatin in it.











Conclutions:
We concluded that even though our hypothesis was correct, our lab is simple and does not even answer the right questions. We spilled a little bit of protease, which could have affected the outtake of it. We also were very one sided in our research, we didn’t test it in different conditions, which made the whole experiment easy and simple. We knew that protease works better in warm temperatures, so we could have worked with it their but we decided to keep things simple, and we kept them a little too simple. Our lab sucked.
Further experiments:
We could have put the gelatin into different temperatures, or into different conditions, their was a lot with this lab that we could have done more with, with a little more experience.
Bibliography:
Peter hoang: laboratory partner, assistance with laboratory
Kirk brown: help with questions, although he did give us those misleading questions we forgive him.
Biology, fifth edition by Cambell, Reece, and Mitchell
www.science-project.com <http://www.science-project.com> - where we got the idea for our lab.