With basis in the in the initial studies of Catanzaro-Guimaraes SA, Catanzaro Guimaraes BP Garcia RB, Alle N, 1986  as well as in the dissertation work of Hidalgo and Consolara (http://www.teses.usp.br/teses/disponiveis/25/25136/tde-21032005-084631/  ), who researching the process of dental reabsorption concluded that process would be caused for the  liberation of sequestered antigen inside organic matrix of dentin, defended at the Bauru School of Dentistry at the Sao Paulo University (FOB-USP), I strongly suggest that dentin matrix of intact teeth may be used  in the bone regenerative therapy as a scaffold bone, conjunctly with stem cells, such as undifferentiated  cells of dental pulp, because among other mechanisms, demineralization promotes the liberation of proteins sequestered in dentin, the example of DMP-1 and LMP-1, which are able to promote to differentiation from stem cells into osteoblasts. Due to the structural, mineral and mechanic similarities between dentin and bone that type of natural scaffold bone may possess advantage in relation to others contend HAs, considering that the process of mineralization of this type of scaffold bone may be easily modified to improve mechanic proprieties as stiffness, toughness and bending strength. The decreased antigenicity of organic dentin matrix resultant of the denaturation proteic by EDTA may be involved in the process of orientation of the mineralization. For testing the "possible" presence of human resorptive dentin antigen, its final antigenicity as well as the presence of growth factors suggested the extraction of the residual proteins in demineralized dentin with guanidinium HCL/EDTA. So, in the prepare of scaffold bone, we suggested the utilization of a demineraliador substances (NaOCL (1%) +EDTA (17%)) to demineralization of the matrix organic of the coronal dentin of a intact tooth.

Antonio Schütz, Ph.D.

Dentist, Pharmacist, Master in Oral Pathology (UFRJ), Doctorate in Oral Pathology (FOB-USP).