Curriculum Vitae

 
2007 - present Scientist at F. Hoffmann-La Roche Ltd., Basel, Switzerland. Pharmaceutical Division, Discovery Enabeling Sciences. Topic: High-Content Screening, Image-based drug discovery, Image Processing, Biostatistics.
2005 - 2007 Diploma of advanced studies in applied statistics, Seminar for Statistics, Department of Mathematics, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland. Thesis: The p-value distribution in genome expression profiling.
2003 - 2007 Post-doc with Horst Vogel, Laboratoire de chimie physique des polymères et membranes (LCPPM), Institut de Sciences et Ingénierie Chimiques (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. Topic: Single-molecule studies of signaling proteins, i.e. G-protein coupled receptors and hormone receptors in living cells; Multiparameter Fluorescence Spectroscopy of single molecules (time-, spectrally, and polarization-resolved single-photon counting).
2001 - 2002 Post-doc in the nano-optics group of V. Sandoghdar, Physical Chemistry Laboratory, ETH Zürich, Switzerland. Topic: Single-molecule studies of the functional dynamics of the molecular chaperone Hsp70/DnaK.
1998 - 2001 Graduate student in the group of U.P. Wild, Physical Chemistry Laboratory, ETH Zürich, Switzerland. Projects: single molecule identification; optical polarization tomography of single molecules; dynamics of single membrane proteins. Thesis title: Multi-parameter Fluorescence Spectroscopy: Illuminating single proteins [Reprint - PDF] (7.34MB)
1997 - 1998 Diploma in physics. Thesis: Force spectroscopy on single polymer molecules with optical tweezers. The work was supervised by E.-L. Florin and done in the Light Microscopy Group of E.H.K. Stelzer and the Local Probes Group of J.K.H. Hörber at the European Molecular Biology Laboratory (EMBL) Heidelberg, Germany.
1995 - 1996 Study of physics at Edinburgh University, Scotland (non-graduating) 
1994 - 1998 Study of physics at Heidelberg University, Germany (final degree)
1992 - 1994 Study of physics at Regensburg University, Germany (first degree)

Research interests 

Biophysics on the level of one or a few molecules;
Chemomechanics of molecular machines like motors, chaperons, receptors, channels, transporters;
Protein synthesis and folding pathways;
Cell signaling at the single molecule level;
Multi-parameter fluorescence microscopy;
Single-molecule manipulation;
Single-molecule detection in nanostructures;
Single-molecule analytics, high throughput and high-content screening applications.
 

Teaching experience

Supervising 3 graduate and 2 undergraduate students.
Lecture on "Introduction to statistical data analysis" for undergraduate students in physical chemistry.
Lecture on “Single-molecules on living cells” for graduate students.
Seminar and lecture series on “Nanobiotechnology and Biophysics” for undergraduate and graduate students in physics, chemistry and biochemistry.
Tutorials and laboratory courses in physical chemistry and physics.

 

Publications

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12. Bulk measurements cannot probe the survival time distribution of a fluorophore
Michael Prummer and Matthias Weiss
Physical Review E 74, 021115 (2006). [Full Article - PDF] (263K)

11. Visualizing odorant receptor trafficking in living cells down to the single-molecule level
Valérie Jacquier, Michael Prummer, Jean-Manuel Segura, Horst Pick, and Horst Vogel
Proceedings of the National Academy of Science (USA) 103, 14325-14330 (2006). [Full Article - PDF] (1'994K)

10. Multifunctional Lipid/Quantum Dot Hybrid Nanocontainers for Controlled Targeting of Live Cells
Gopakumar Gopalakrishnan, Christophe Danelon, Paulina Izewska, Michael Prummer, Pierre-Yves Bolinger, Isabelle Geissbühler, Davide Demurtas, Jacques Dubochet, and Horst Vogel
Angewandte Chemie International Edition 45, 5478-5483 (2006). [Full Article - PDF] (407K)

9. Posttranslational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells
Michael Prummer, Bruno H. Meyer, Raphael Francini, Jean-Manuel Segura, Nathalie George, Kai Johnsson, and Horst Vogel
ChemBioChem 7, 908-911 (2006).[Full Article - PDF] (189K)

8. Diffusion-time distribution analysis reveals characteristic ligand-dependent interaction patterns of nuclear receptors in living cells
Hanna Jankevics, Michael Prummer, Paulina Izewska, Horst Pick, Kirsten Leufgen, and Horst Vogel
Biochemistry 44, 11676-11683, (2005). [Full Article - PDF] (232K)

7. Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics
Michael Prummer*, Beate Sick*, Alois Renn, and Urs P. Wild
Analytical Chemistry 76, 1633-1640 (2004). [Full Article - PDF] (336K)

6. Three-Dimensional Optical Polarization Tomography of Single Molecules
Michael Prummer, Beate Sick, Bert Hecht, Urs P. Wild
Journal of Chemical Physics 118, 9824-9829 (2003). [Full Article - PDF] (853K)

5. The citrate carrier CitS probed by single-molecule fluorescence spectroscopy
Christopher N. Kästner*, Michael Prummer*, Beate Sick*, Alois Renn, Urs P. Wild, and Peter Dimroth
Biophysical Journal 84, 1651-1659 (2003). [Full Article - PDF] (289K)

4. Orientation dependence of fluorescence lifetimes near an interface
Maximilian Kreiter, Michael Prummer, Bert Hecht, and Urs P. Wild
Journal of Chemical Physics 117, 9430-9434 (2002). [Full Article - PDF] (144K)

3. Coupled rotation within single F0F1 enzyme complexes during ATP synthesis or hydrolysis
Georg Kaim*, Michael Prummer*, Beate Sick*, Gert Zumofen, Alois Renn, Urs P. Wild, and Peter Dimroth
FEBS Letters 525, 156-163 (2002). [Full Article - PDF] (560K)

2. Single-Molecule Identification by Spectrally and Time-Resolved Fluorescence Detection
Michael Prummer, Christian G. Hübner, Beate Sick, Bert Hecht, Alois Renn, and Urs P.Wild
Analytical Chemistry 72, 443-447 (2000). [Full Article - PDF] (128K)
Note the misprint in eq. 4: both summations over the photon number j need to be replaced by products.

1. Three-Dimensional High-Resolution Particle Tracking for Optical Tweezers by Forward Scattered Light
A. Pralle, M. Prummer, E.-L. Florin, E.H.K. Stelzer, J.K.H. Hörber
Microscopy Research and Techniques 44, 378-386 (1999). [Full Article - PDF] (375K)

*These authors contributed equally to this work.
 

In the public press

"Single-molecule analytical method measures 14 parameters" by Kevin Robinson,
Biophotonics International,  May 2004, Laurin Publishing Co., Inc., Pittsfield MA.

"Fluorescence correlation spectroscopy technique adds quantitative details" by Gary Boas,
Biophotonics International,  November 2005, Laurin Publishing Co., Inc., Pittsfield MA.

Patent

“Method for testing a substance interacting with a target molecule”,
Hanna Jankewics, Michael Prummer and Horst Vogel,
WO2006013109 (09 Feb 2006, priority date 06 Aug 2004).

Talks

Biophotonics - shedding light on single proteins
Colloquium, invited by Lluis Torner, Institut de Ciences Fotoniques (ICFO), 23.11.2007, Barcelona, E.

Protein mobility in cell signaling reactions at the single molecule level
Colloquium, invited by Thilo Enderle, High Throughput Screening, F. Hoffmann-La Roche Ltd., 29.06.2007, Basel, CH.

Cell signaling at the single molecule level (invited)
Latsis Symposium, 25.-27.06.2007, EPFL, Lausanne, CH.

Protein mobility in cell signaling at the single molecule level
Seminar, invited by Christian Hübner, Institut für Physik, 04.04.2006, Universität Lübeck, D.

Mobility and signaling of single membrane receptors in living cells
Biophysics Club, Institute of Physics of Complex Matter, 19.04.2007, EPFL, Lausanne, CH.

Tracking the scent: functional mobility of odorant receptors in living cells at the single molecule level
Michael Prummer, Valérie Jacquier, Jean-Manuel Segura, Horst Pick, and Horst Vogel
Platform presentation at the 51th Annual Meeting of the Biophysical Society, 03.-07.03.2007, Baltimore, MD, USA.

Protein motion in the cell membrane
LCPPM winter school, 13.02. - 16.02.2007, Leukerbad, CH.

Single-molecule microscopy and spectroscopy of biological nanomachines
Invited colloquium, DFG Research Center for Functional Nanostructures, Karlsruhe University of Technology, 09.02.2007, Karl-TECH, Karlsruhe, D.

Zelluläre Signalpfade und Nanomechanik einzelner Proteine
Colloquium, invited by Werner Mäntele, Institut für Biophysik, 31.01.2007, Universität Frankfurt, D.

Cellular reactions by single-molecule spectroscopy (invited)
Photonics Day, NCCR Quantum Photonics & Swiss Optical Society, 24.11.2006, EPFL, Lausanne, CH.

Cell signaling and nanomechanics of single proteins
Colloquium, invited by Klaus Meerholz, University of Köln, and Benjamin Kaupp, Jülich Research Institute, 18.09.2006, Jülich, D.

LSM 510 Meta Confocor 3 (instrument demonstration)
3rd Zeiss Live Cell Imaging Workshop, Center for Integrative Genomics, 10.05.2006, Lausanne, CH .

Illuminating biological nano-machines with single-molecule spectroscopy
Colloquium, invited by Ian Campbell, Department of Biochemistry, 10.01.2006, Oxford University, UK.
Seminar talk, invited by Matthias Weiss, German Cancer Research Institute (DKFZ), 04.04.2006, Heidelberg, D.

Single Molecule Spectroscopy: Illuminating Protein Dynamics and Interactions
Seminar talk, invited by Petra Schwille, Institut für Biophysik/BioTec, 13.06.2005, TU Dresden, Germany,
Colloquium, invited by Jörg Wachtrupp, 3. Physikalisches Institut, 05.07.2005, Universität Stuttgart, Germany.

Elucidating cellular signalling by imaging single molecules (invited, representing Horst Vogel)
Bi-annual meeting of the German Biophysical Society, 05.05.-08.05.2005, Hünfeld, Germany.

Multi-Parameter Fluorescence Spectroscopy: Illuminating Single Biomolecules
Colloquium, invited by Wolfgang Maier, Institute of Physical Chemistry, 05.04.2005, University of Basel ,Switzerland.

Single Molecule Fluorescence Spectroscopy: Elucidating Protein Dynamics and Interactions
Colloquium, invited by Thomas. Kiefhaber, Biozentrum, 19.04.2005, University of Basel, Switzerland.

Tracking of individual G-protein coupled receptors on living cells
Michael Prummer, Bruno H. Meyer, Raphael Francini, Jean-Manuel Segura, and Horst Vogel
Talk given at the annual meeting of the German Physical Society (DPG), 04.-09.03.2005, Berlin, Germany.

Investigating nuclear receptor interactions in vivo with diffusion time distribution analysis
Michael Prummer, Hanna Jankevics, Paulina Izewska, Horst Pick, Kirsten Leufgen, and Horst Vogel
Platform presentation at the 49th Annual Meeting of the Biophysical Society, 12.-16.02.2005, Long Beach, USA.

Monitoring individual neurokinin-1 receptors on living cells
Talk given at the LCPPM autumn school “Biomolecular interactions on the micro- and nanometer scale”,
20.09.-24.09.2004, Rosenlaui, Switzerland.

Optical Polarization Tomography: A new method to determine the three-dimensional orientation of single molecules
Michael Prummer, Horst Vogel, Beate Sick, Bert Hecht, Urs P. Wild
Talk given at the annual meeting of the German Physical Society (DPG), 08.-12.03.2004, Regensburg, Germany.

Probing individual F0F1 ATP synthases by multi-parameter fluorescence spectroscopy
Michael Prummer, Horst Vogel, Beate Sick, Alois Renn, Gert Zumofen, Urs P. Wild, Georg Kaim, Peter Dimroth
Talk given at the annual meeting of the German Physical Society (DPG), 08.-12.03.2004, Regensburg, Germany.

Multi-parameter fluorescence spectroscopy of single molecules: Take everything a photon can give
Seminar talk, invited by M. Arndt and A. Zeilinger, Institute for Experimental Physics, 04.02.2003, Vienna University, Austria.
Physikalisches Kolloquium, invited by M. Radmacher, Institut für Biophysik, 21.10.2003, Universität Bremen, Germany.

Multi-parameter confocal microscopy and time-resolved emission spectroscopy of single molecules
Michael Prummer, Beate Sick, Alois Renn, Urs P. Wild
Talk presented at the 5th Annual Winter Workshop, 31.01.-03.02.2003, Linz, Austria.

Illuminating Single Proteins by Multi-Parameter Fluorescence Spectroscopy
Seminar talk, invited by M. Auer, Novartis Research Institute, 11.10.2002, Vienna, Austria.

OBSERVING SINGLE F0F1 ATP SYNTHASES AT WORK
Michael Prummer, Beate Sick, Alois Renn, Urs P. Wild, Georg Kaim, Christopher Kaestner, Peter Dimroth
Platform presentation at the 46th Annual Meeting of the Biophysical Socienty, 23.-27.02.2002, San Francisco, CA.
Seminar talk, invited by J. Crain and P. Ghazal, COSMIC seminar & GTI, 10.02.2003, Edinburgh University, UK

Observing single F0F1 during ATP synthesis
Michael Prummer, Beate Sick, Alois Renn, Urs P. Wild, Georg Kaim, Christopher Kaestner, Peter Dimroth
Seminar talk, invited by T. Nilssen, European Molecular Biology Laboratory (EMBL), 16.08.2001, Heidelberg, Germany.

Three-Dimensional Optical Polarization Tomography of Single Molecules
Michael Prummer, Bert Hecht, Urs P. Wild
Talk given at the Sci & Ski Seminar 2000, 20.03.-24.03.2000, Engelberg, Switzerland, organized by the Wild-Group.

Prospects in Single Molecule Biochemistry
Seminar talk, invited by F.U. Hartl, Cellular Biochemistry,  Max-Planck-Institute for Biochemistry, 13.08.1999, Martinsried, Germany,
Seminar talk, invited by B. Bukau, Biochemistry & Molecular Biology, 17.04.2000, Freiburg University, Germany.

The Elasticity of Single Molecules and 3d Scanning Probe Microscopy - a Task for Optical Tweezers
M. Prummer, E.-L. Florin, E.H.K. Stelzer, J.K.H. Hörber
Seminar talk, invited by T. Geisel, Non-linear dynamics group, Max-Planck-Institute for Fluid Dynamics, 13.09.1999, Gottingen, Germany.

Selected Posters

Tracking the scent: functional mobility of odorant receptors
Michael Prummer, Valerie Jacquier, Horst Pick, Horst Vogel
Frontiers in Chemical Biology: Single Molecules, The Royal Society of Chemistry, 26.-29.03.2006, Cambridge, UK.

Monitoring individual odorant receptors in cultured mammalian cells
Michael Prummer, Valerie Jacquier, Horst Pick, Horst Vogel
Annual meeting of the German Physical Society (DPG), Biological Physics, 04.-09.03.2005,Berlin, Germany.

Investigating hormone receptor interactions in vivo with diffusion time distribution analysis
Michael Prummer, Hanna Jankevics, Horst Vogel
NRP 50 annual meeting “Endocrine Disruptors: Relevance to Humans, Animals and Ecosystems“,
10.-12.10.2004, Gurten, Bern, Switzerland.

Single-molecule microscopy of the rotary motor F0F1 ATP synthase at work
Michael Prummer, Horst Vogel, Beate Sick, Alois Renn, Gert Zumofen, Urs P. Wild, Georg Kaim, Peter Dimroth
Poster presented at the annual meeting of the German Physical Society (DPG), 08.-12.03.2004, Regensburg, Germany.

Investigating structure and function of single citrate transport proteins
Michael Prummer, Horst Vogel, Beate Sick, Alois Renn, Urs P. Wild, Christopher Kaestner, Peter Dimroth
Poster presented at the annual meeting of the German Physical Society (DPG), 08.-12.03.2004, Regensburg, Germany.

Multi-parameter fluorescence spectroscopy of single dye-conjugated F0F1 ATP synthases
Michael Prummer, Beate Sick, Georg Kaim, Alois Renn.
Poster presented at the International Symposium "Elucidating biomolecular networks by single-molecule technologies", 26.-31.10.2003, Monte Verita, Ascona, Switzerland.

Studying structure and function of single membrane transport proteins by fluorescence quenching and dual-color colocalization
Michael Prummer, Beate Sick, Alois Renn, Urs P. Wild, Christopher N. Kästner, and Peter Dimroth
Poster presented at the 5th Annual Winter Workshop “Single Molecule Techniques in Biophysics and Drug Discovery”, 31.01. - 03.02.2003, Linz, Austria.

Observing single F0F1 ATPases at work
Michael Prummer, Beate Sick, Alois Renn, Urs P. Wild, Georg Kaim, Christopher Kaestner, Peter Dimroth
Poster presented at the 4th International Conference on Biological Physics, 30.07.- 03.08.2001, Kyoto, Japan, at the 10th International Conference on Unconventional Photoactive Systems, 04. - 08.09.2001, Les Diablerets, Switzerland, aand at the WE-Heraeus-Seminar 282 "Single Molecule Dynamics", 18. - 21.06.2002, Bad Honnef, Germany.

Three-Dimensional Optical Polarization Tomography of Single Molecules
Michael Prummer, Bert Hecht, Urs P. Wild , European Biophysics Journal 29, 383 (2000)
Poster presented at the 3rd European Biophysics Congress, 09.09.-13.09.2000, Munich, Germany, organized by the European Biophysical Societies' Association (EBSA) and the German Biophysical Society, and at the 2nd International Symposium on Physics, Chemistry and Biology with Single Molecules 05.-07.03.2001, Kloster Banz, Germany, organized by the Volkswagen Stiftung.

MULTIDIMENSIONAL PHOTON COUNTING AND SEQUENTIAL ANALYSIS FOR RAPID IDENTIFICATION OF SINGLE MOLECULES IN AMBIENT CONDITIONS
M. Prummer, C.G. Hübner, B. Sick, B. Hecht, A. Renn, U.P. Wild
Poster presented at the 5th Single Molecule Detection Workshop, 29.09.-01.10.1999, Berlin organized by PicoQuant GmbH, and at the 6th Minerva Winter School on Physical Aspects of Biological Systems, 27.02.-03.03.2000, Weizmann Institute of Science, Rehovot, Istrael.

Hochauflösende dreidimensionale interferometrische Positionsdetektion in der Photonischen Kraftmikroskopie
M. Prummer, A. Pralle, E.-L. Florin, E. H. K. Stelzer und J. K. H. Hörber
Poster presented at the workshop "Scanning-Probe-Microscopies and Organic Materials VII, 07.-09.10.1998, Berlin, Germany.
 
 

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