ROP BIOTECHNOLOGY POLICIES & COURSE CURRICULUM 2007-2008

Goals, Objectives and Curriculum Outline

ROP Instructor: Mr.Hudson

Web site: http://www.geocities.com/mhudsonj/BiotechnologyClass.html

Email: [email protected] Phone: 510-231-1429 ext 25224

Goals:

Students will have basic knowledge of the theories underlying biotechnology:

►             DNA Cloning

►             Analysis of Cloned DNA

Students will know practical applications of DNA Technology:

►             Medical, pharmaceutical, forensic, environmental and agricultural

Students will be able to perform various applications and techniques for problem solving. Students will know how to obtain jobs in biotechnology industry, and will be given practical biotechnology laboratory skills for use in an industrial setting.

Grading Policy

              Your overall grade will depend on completing all the assignments. The grade will be broken down into the following categories:

     60% Laboratories

              These will be broken down into experiments and lab techniques. Your lab book will count as 50% of the lab grade. Attendance, completion of the labs as well as the level of quality your work exhibits will be calculated into the rest of your laboratory grade. This is a very important component of your progress in this class.

     40% Activities

              You will be performing numerous online activities, allowing you to complete the course topic outline. Those activities done in class will include fun hands-on modeling activities, research projects and presentations. To ensure you have completed the activities, I’ll be giving quizzes to check that all of you have completed the assignments.

     Grades are based on the following percentages:

       91% to 100% is an A, 90.9% to 89.6% is an A-

       89.5% to 87% is a B+, 86.9% to 81% is a B, 80.9% to 79.0% is a B-

       78.9% to 77% is a C+, 76.9% to 71% is a C, 70.9% to 67% is a C-

       66.9% to 61% is a D, 60.9% to 57% is a D-

       56.9% or below is an F (We need to talk about your future)

Objectives

        The course will provide a challenging learning environment. Students will participate in research projects, as well as learning job skills. For the Biotech students, the table on the next page will give you an idea of the activities you all will perform.

Safety and Lab Protocol

       All of you will learn your way around the lab. Labs are clean, well maintained areas of work. Since you are in a vocational class, I’ll be giving you training on the proper use of equipment and tools. When working with different techniques, you will need to use another set of skills and protocols to accomplish your work. Following instructions will make your work in the lab safe for you as well as all the other students around you.

LAB SCHEDULE FOR 2008-2009

For Biotechnology Students

SPECIFIC LAB SKILLS:

Use Of Micropipettes                                                  Preparation Of Liquid And Gel Media

Aseptic Techniques                                                     Bacterial Streaking To Single Colonies

Inoculation Of Liquid Cultures                                  Dilution Series/Plating

Sterile Transfers                                                           Protein Purification

Bacterial Transformations                                          Preparing Solutions

Agarose Gel Preparation                                             Agarose Gel Analysis

Restriction And Ligation Enzyme Use                      Preparation Of Buffers

Protein Extraction                                                       Uses Of Column Chromatography

Use Of Bioinstrumentation