Review Questions Dec 04

1. Plasmids are ______.

A. repeating sequences that protect DNA from digestion when it is inserted into a foreign cell

B. small circlets of DNA found in bacteria

C. segments of RNA that must be attached to DNA before the DNA can replicate

D. ends of cut DNA molecules that are "sticky" because they have unpaired base sequences

E. infectious proteins

 

2. DNA used in recombinant DNA techniques is first cut into fragments by _____.  The DNA fragments are then joined together by ______.

A. restriction enzyme........a DNA polymerase

B. a DNA ligase.................restriction enzymes

C. a reverse transriptase...a DNA ligase

D. restriction enzymes.......a DNA ligase

E. UV irradiation................a DNA polymerase

 

3. Which of the following techniques are useful in identifying DNAs from traces of blood in a crime scene?

A. polymerase chain reaction (PCR)

B. genetic transformation

C. gene cloning

D. reverse transcription

E. signal transduction

 

4. Which of the followings is an example of genetically modified organisms?

A. a human treated with insulin produced by E. coli bacteria

B. a disease resistant rice variety produced via traditional breeding

C. a carrot plant regenerated from a single somatic cell.

D. an insect resistant potato variety carrying the bacterial Bt toxin gene

E. a human who received a corrected cystic fibrosis gene via gene therapy

 

5. A human cell contains about 1000 times more DNA than an E. coli cell.  However, the number of genes in the human genome is only 40 times more than the E. coli genome.  Why?

A. Because human cells are much larger than E. coli cells.

B. Because human body consists of many cells.

C. Because human cells differentiate into many different types of cells.

D. Because a majority of the human genome consists of ‘junk’ sequences.

 

6. The figure below is DNA fingerprint patterns from a murder victim, suspect 1, suspect 2 and a mixed blood sample from the crime scene.  Which suspect is more likely to have committed the crime?

       Victim     Suspect 1   Suspect 2    Crime scene

 

7. The figure below is a DNA fingerprint analysis to determine paternity of children.  Which child is NOT genetically related to the father shown here?

 

 

 

 

 

 

 

 

 

 

 

8. Identify a genetic marker that are inherited together with the disease X in the figure above.

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