Accession Number
039054070
Authors
Yuan W. Hooten C D. Lezica R P. Showalter
A M.
Institution
BOTANY DEP., MOL. AND CELLULAR BIOL.
PROGRAM, OHIO UNIV., ATHENS, GA. 45701.
Title
CLONING AND CHARACTERIZATION OF COMPLEMENTARY
DNA ENCODING POTATO TUBER LECTIN AND EXTENSIN.
Source
ANNUAL MEETING OF THE AMERICAN SOCIETY
OF PLANT PHYSIOLOGISTS, INDIANAPOLIS, INDIANA, USA, JULY 29-AUGUST 2, 1990.
Plant Physiology
(Bethesda) 93 (1 Suppl.). 1990. 42.
Year of Publication
1990
Publication Type
Meeting.
ISSN
0032-0889
Keywords
ABSTRACT.
Concept Codes
*Genetics and Cytogenetics / Plant [03504]
*Biochemical Studies / Nucleic Acids,
Purines and Pyrimidines [10062]
*Biophysics / Molecular Properties and
Macromolecules [10506]
*Metabolism / Carbohydrates [13004]
*Metabolism / Proteins, Peptides and
Amino Acids [13012]
*Plant Physiology, Biochemistry and
Biophysics / Metabolism [51519]
General Biology / Symposia, Transactions
and Proceedings of Conferences, Congresses, Review Annuals [00520]
Biochemical Studies / Proteins, Peptides
and Amino Acids [10064]
Biochemical Studies / Carbohydrates
[10068]
Biosystematic Codes/Super Taxa
Solanaceae [26775]
Plants. Vascular Plants. Spermatophytes.
Angiosperms. Dicots.
Languages
English.
Abstrate
The solanaceous lectins represent a
class of plant hydroxyproline glycoproteins which bind oligomers of N-acetylglucosamine.
Potato tuber lectin (PTL) has been found in potato tuber and is biochemically
the best studied of the solanaceous lectins. Potato tuber lectin
consists of two distinct domains: 1) a glycosylated domain, like plant
cell wall extensin, rich in hydroxyproline (Hyp) and serine with 1-4 arabinoses
linked to Hyp and 1 to 2 galactoses O-linked to serine and 2) a non-glycosylated
domain, like wheat germ agglutinin (WGA) and other potato cysteine-rich
proteins, rich in glycine and cysteine and capable of binding oligomers
of N-acetylglucosamine. These lectins are thought to function in
cell-cell recognition, wound healing, and plant defense since N-acetylgluco-samine
is a major component of cell wall. No amino acid sequences nor gene
information for the hydroxyproline-rich solanaceous lectins are published.
We are trying to isolate and characterize potato tuber lectin cDNA clones.
This research will provide preliminary materials and information necessary
for further isolation of plant genes encoding this group of lectins and
related proteins such as some cysteine-rich phloem proteins, for studies
on regulation of these genes, and for the investigation of solanaceous
lectin function via genetic engineering approaches.
Entry Week
9000
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