Accession Number
091054616
Authors
Yuan W.
Institution
DEP. BOT., OHIO UNIV., ATHENS, OHIO
45701, USA.
Title
CULTURE MEDIUM FOR XANTHOMONAS-CAMPESTRIS
PATHOVAR ORYZAE.
Source
Journal of Applied Bacteriology 69 (6).
1990. 798-805.
Year of Publication
1990
Publication Type
Article.
ISSN
0021-8847
Keywords
RICE LEAF BLIGHT PATHOGEN ERWINIA-HERBICOLA
TRYPTONE PEPTONE NUTRIENTS.
Concept Codes
*Physiology and Biochemistry of Bacteria
[31000]
*Microbiological Apparatus, Methods
and Media [32000]
*Phytopathology / Diseases Caused by
Bacteria [54504]
Biochemical Studies / General [10060]
Biochemical Studies / Proteins, Peptides
and Amino Acids [10064]
Biochemical Studies / Carbohydrates
[10068]
Biochemical Studies / Minerals [10069]
Nutrition / General Studies, Nutritional
Status and Methods [13202]
Nutrition / Minerals [13206]
Nutrition / Carbohydrates (1972- ) [13220]
Nutrition / Proteins, Peptides and Amino
Acids (1972- ) [13224]
Biosystematic Codes/Super Taxa
Pseudomonadaceae (1979-91) [04716]
Enterobacteriaceae (1979-91) [04810]
Gramineae [25305]
Microorganisms. Bacteria. Plants. Vascular
Plants. Spermatophytes. Angiosperms. Monocots.
Languages
English.
Abstract
Studies on nutrient requirements of
four Chinese strains of Xanthomonas campestris pv. oryzae in a modified
Watanabe's medium led to the development of a new synthetic
medium containing sucrose, sodium glutamate,
methionine, KH2PO4, NH4Cl and iron chelated with EDTA. The concentration
of each ingredient was optimized based on the
number of colonies and time required
for their appearance. Various concentrations of some nutrients were compared
based upon their effects on growth of the pathogen strains
and 34 contaminants from rice materials.
Tryptone enhanced the growth of X. c. oryzae more than that of many contaminants,
including Erwinia herbicola. Peptone stimulated
growth of X. c. oryzae without promoting
excessive contamination. When compared with other media used for X. c.
oryzae, the new culture medium enriched with tryptone and
peptone gave the highest recovery and
earliest appearance of colonies of Chinese strains of this bacterium.
Entry Week
9100
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