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Edge Forums Strains & Hybridization Breeders Lab Question about the Linnaean nomenclature of Cannabis sativa, indica, and ruderalis.
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Green Shaman
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Green Shaman

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   GreenHeart,
Yes I believe the seed type is the most relevant "trait" to tell a "Sativa" from an "Indica", and as far as I can tell, this never changes in a seed line unless you cross a "Sativa" with an "Indica" (the offspring can then produce "Sativa"-type seeds, or "Indica"-type seeds, or something in-between -- the latter being very common). In a seedline that is also an IBL, and either pure "Sativa" or pure "Indica", the colour of all the seeds from all the female plants of a batch will be pretty much the same (same kind of stripes too, if any). The size of seeds may (will) vary, but that's usually the only difference.

Now, with S/I hybrids, especially F1s, the type of seeds as well as the phenotypes aren't relevant. Because a hybrid could be Sativa dominant from a genetical point of view, and show mostly "Indica" traits, including the type of seeds. In such a cross the "Indica" would be dominant as far as phenotypes are concerned. This could just be due to the fact that one of the selected parents for a F1 seedline happened to be "Indica" dominant, or breeds true for these traits, while there could have been many other, more "Sativa" looking plants of the same strain, same generation, but these were not used in the end. A commercial F1 hybrid is only, after all, the offspring of one selected male and one selected female, each parent being only one phenotype (or rather, phenotypic group) among several.


Strawdog,
Granted, the genes, or alleles, aren't changed by environmental conditions. The drift toward different phenotypes could, in theory, be reversed (it should work both ways). However, I believe that lost phenos are... lost.
I mean, oriental short, bushy hash plants grown for generations in NW Europe will tend to get taller, less bushy, and less resinous. In this different environment (Europe), the plants will produce more fibers and less resin after a few generations. I am not sure, however, that if you bring this acclimatized type "back" in its original country, it will reverse fully to its former state. Obviously, I have never reintroduced in the wild seedlines I've grown for generations. Observations on the field make me think it doesn't work the other way round, though. Like, the escaped plants in North Africa never reverted to a real "resinous Indica" type, even after a hundred years, despite favorable conditions...

Greenz,
There won't be a genetical change. I never said there would be. There will be a difference in phenotypes, and that's all we can see (nobody's working on Cannabis DNA mapping here, I guess?). BUT that's also all we can use to differenciate "Sativas" from "Indicas" since there IS NO proven genetical difference, at least nothing sufficient to permit a split between two distinctive species of Cannabis or more.
And again, this "Sativa" vs "Indica" phenotypes talk is futile, since the traits commonly mentioned to differenciate one type from the other aren't very relevant since some of them can't be used for some geographical types.

PIC:I believe I MUST post a pic that will illustrate my point of view better. So here's an original pure Indica, an Afghan cultivar in Mazar-e-Sharif. Supposedly a pure Indica hash plant, then. I'll let you check the type of leaf, internodal distance, and floral cluster type (from what you can guess on that pic).

Last edited by Green Shaman on November 9th, 2004 at 01:05 PM

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strawdog
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Green Shaman,

Afghanistan has both Indica and Sativa landrace and hybrids of both. You can find wild Sativa in Afghanistan and the same in India.

Technically the species never crossed in the wild due to isolation but the locals bred them together for Hash making.

In fact, typically, Afghanistan was a Sativa Hashish producer before it became a Indica Hashish producer by mixing the two. As you can see in your picture you have what looks like a Mostly Indica or even an Indica/Sativa hybrid. We really need to see a population to know more.

The are many species types in Afghanistan including Ruderalis as you go north towards the South of Russia.


 

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Last edited by strawdog on November 9th, 2004 at 01:31 PM

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Greenz
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Greenz

Registered: Nov 2001
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Originally posted by Greenz
However, I think (and possibly c-ray was implying) that cannabis may be able to evolve in a matter of a few hundred years.


Green Shaman's reply:

"A few hundred years? A couple generations, you mean."

But then after he said:
"There won't be a genetical change. I never said there would be. There will be a difference in phenotypes, and that's all we can see (nobody's working on Cannabis DNA mapping here, I guess?). BUT that's also all we can use to differenciate "Sativas" from "Indicas" since there IS NO proven genetical difference"

Alright,
Of course, by evolve, I meant an ACTUAL genetic change. I mean, I'm pretty sure everyone already knows that environment can affect the resulting phenotypes that are expressed. That's a given. My point is that if you took seeds of indica origin, then bred them only for a couple generations in an equatorial region, the resulting seeds would not have changed that quickly to a sativa variety. It's as simple as that. They might appear more sativa, but that's only the environments effect on morphology. What C-ray and I were talking about was an real genetic change from indica to sativa after MANY generations of being bred in an equatorial environment.

Consider this scenerio (expanded version of my old scenerio):
You've got some "indica" seeds. You live for 200 years. LOL. When you are young, you decide you want to breed these seeds outside near the equator. First, however, you grow a batch of these seeds inside and take note on the physical structure of the plants (pictures) and also the flowering time, potency etc... They appear to be the "typical indica".Then you start breeding them outside. You do this for 150 years. However, after 3 generations you notice that during these years, the plants outside don't look exactly the same as the ones you grew inside three years before. So, you take a batch of seeds from that years crop and grow them inside to compare to the batch you grew the first year. However, the resulting plants still appear quite similar to the "indica" plants that you grew three years earlier inside. You realize it was mostly just the environment affecting morphology of your plants outside. You continue breeding them outdoors. After 150 years, you notice that your outdoor crop really looks very different then it did when you first started breeding them. They are taller, lankier, and take longer to finish, and have a more pychoactive high. So, you take some seeds from the last years crop and decide to grow them inside again. You grow them inside and take pictures and take note of the flowering time etc... This time you notice a big difference when you compare the pictures to the plants from 150 years ago. You grew them exactly the same indoors as you did 150 years ago, but the plants look very different. Now, they are taller, lankier, and take much longer on 12/12 to finish. Here, there has been an ACTUAL genetic change.
Make sense?


Also,
There obviously is a genetic difference between indicas and sativas, otherwise there morphology would be the same if they were grown in the exact same environment (which we know from indoor growing). When grown indoors, varieties of sativa origin appear sativa, and ones of indica origin appear indica.

Alright Later guys,

Greenz


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Green Shaman
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Okay, here we go again (I think I begin to like this thread too ).

Strawdog,
Thanks, I know there are alpine varieties as well as "lowland" varieties in Afghanistan. You're wrong there, though, as regards the non-interbreeding of both types. You will find broad-leave plants only in gardens where selective pressure is applied, or where there is no slim-leaved plants growing wild nearby. The slim-leaved plants are pulled, is all. "Afghani" refers to so many types of plants, really...
And the Himalayan Sativas (wild or cultivated) aren't so different from some "lowland" types. Check the famous Nepali Sativa, that is used as a hash plant. Except the long internodes and meagre yield, it looks very "Indica", don't you think? It produces less resin, and the hash is less "oily", more "dry". But it has the typical taste and smell of all the hash produced in the region. There are also lots of slim-leaved plants in high valleys between India and Pakistan. These are very resinous and are used to produce hash as well, so they're considered "Indica". But technically, they're very different from the plants on the other side of the mountains, like the Citral valley, for instance.

Greenz,
Once again, my point here is that refering to "Sativa" plants vs "Indica" plants is futile and definitively artificial.


My point is that if you took seeds of indica origin, then bred them only for a couple generations in an equatorial region, the resulting seeds would not have changed that quickly to a sativa variety. It's as simple as that. They might appear more sativa, but that's only the environments effect on morphology. What C-ray and I were talking about was an real genetic change from indica to sativa after MANY generations of being bred in an equatorial environment.


What you mean here is that the drift of an oriental lowland, broad-leaved, short stature type toward a taller, intermediate or slim-leaved type would take time. Correct, but don't call such a strain just "Indica" (as a matter of fact, a good number of lowland, broad-leaved "Sativas" could qualify for these "Indica" traits as well). Because there are LOTS of "Indica" types that are already quite "Sativa"-like, as regards leaf, internodes, critical photoperiod, and possibly other phenotypes. And such plants grown under tropical conditions side by side with local "Sativas", provided the critical photoperiod isn't too different, would certainly look very "local" after a few generations only.

You see, it's all about what we call "Sativa" and what we call "Indica", and each "type" refers in fact to a quite wide range of (geographical and human-induced) variation.

Going from "Indica" to "Sativa":

  • On one extreme we have the lowland, broad-leaved, incredibly resinous hash plants. The "typical Indicas".

  • Then come "intermediate Indicas", the taller, more lanky or still bushy, intermediate-leaved hash plants. I would personally add the North India, North Africa and Middle-East types in this category.

  • The come the "intermediate Sativas", somewhat short, bushy and intermediate-leaved marijuana plants (South Asia, lowland and "jungle" types).

  • Far on the other "end" of the spectrum, we have the slim-leaved, lanky, equatorial and tropical marijuana plants, the "typical Sativas".


It's all very subjective, in fact, and telling an "intermediate Indica" from an "intermediate Sativa" would be impossible in some cases. If the geographical origin is unknown, and if you can't see the seeds or smoke the buds, you can't tell.
Do the test: post pics of phenotypically intermediate landrace cultivars, IBLs, and ask everybody what type they are. It's useless.

Sooo, if I'm not happy with the definitions of "Indicas" and "Sativas" (Ruderalis would be a problem, that's why I haven't mentioned that third type yet), what would be better descriptions?

Well, in my opinion, we should mention a marijuana or hash "drug" Cannabis plant, and its geographical origin. And nothing else.

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strawdog
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There are 3 types of cultivar to consider.

Wild (non-cultivar)
Landrace (semi-cultivar)
Domesticated (cultivar)

Landrace is wild cannabis that has had a certain human influence (selective breeding) but remains somewhat wild because man has let it. True wild species have no direct man-made selective breeding pressures at all. Domesticated plants certainly do.

What you need to do is look at Vavilov's early discoveries in Afghanistan which is why we know so much about Afghanistan's background in Hash production.

He discovered a wild race of Indica in Afghanistan that was not being used by the locals.

Later he discovered that locals decided to breed this Indica with their domestic sativa hash producing plants to produce a new hashish cultivar.

In Afghanistan he found...

Domesticated Sativa
Landrace Sativa
Wild Indica

Later on his discovered...

Domesticated Indica
Indica/Sativa hybrids

Now we have...

Domesticated Sativa
Domesticated Indica
Domesticated Sativa/Indica hybrids
Landrace Indica
Landrace Sativa/Indica hybrids.

And Ruderalis...

All in Afghanistan... much like us except the wild/landrace side of things.


 

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Last edited by strawdog on November 10th, 2004 at 12:31 PM

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Green Shaman
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Several types of cultigens, you mean.

Cultivars are just cultivated plants. They can have a lot in common with wild plants or be very different. They can be stable, true-breeding or not.

There's been a lot of work and interest about Afghan Cannabis and Hashish. Come on, what you mention here has happened naturally and artificially simultaneously or not in lots of different places, not just Afghanistan. If two distinct populations that are also two very different types aren't in contact with each other, as is the case with many valley plants and their alpine neighboors, each type preserves its "typical" traits. But it takes nothing more than a gust of wind to change that, unless one type can really not live and prosper in the other environment. Like, the alpine type can grow in valleys but the lowland type will die up in the mountains.

Also, what do you call a "wild" type, and how do you tell a "wild" indigenous plant from a feral population that escaped from cultivated fields, even if it happened long ago? About when do you consider a population "wild" and not "escaped" anymore?

It is also funny that the work you mention does not refer to "wild Sativa". Does this mean the alpine "Sativas" that grow naturally in lots of places in the Himalayan where no man has cultivated them before, and where cultivated types can't survive, aren't wild plants?

The truth is, there's a lot of BS in all this. I have no personal opinion on Mr Vavilov's work. I also know that there is a "Russian" "school" of scientists, all pleading in favor of a split between at least two distinct subspecies of Cannabis, vs "the rest of the world" scientists, who don't agree. Shall we exchange links regarding Mr Vavilov's studies and how they are questioned, to say the least, by other prominent botanists?

I don't want to sound rude, but Vavilov's work mentions just what I've posted here too. Let me quote him (I hope he won't mind, now): "series of races of the typical wild and typical cultivated forms of hemp show many overlapping characters", and also that all transitional forms from wild hemp to cultivated hemp can be found, and that for many forms "the morphological limits between weed hemp and true wild hemp are difficult to draw, and in regard to the characters of the fruits, the flowers and the leaves, they are indistinguishable from each other".

On the other hand, in a brief treatment dealing with material collected in Afghanistan, Vavilov and Buchinich (1929) recognized C. indica as distinct from C. sativa by small leaves, small fruit, and low growth. And that's all. Interesting, we know more today.

I won't cite the complete thing here, but back in 1926 what Vavilov described met, let's say, considerable disagreement.

Besides, there have been lots of more recent studies that show that there can be more internal variation (chemical constitution) between plants of a same Cannabis "race" or "subspecies" (as described by authors like Vavilov or Schultes) than between plants of different geographical "races".

You should really abandon these concepts of "Sativa" and "Indica", and Vavilov was certainly not the best reference to mention here. Now a debate between Shultes' work and was someone like E. Small had to say about it in 1975 would certainly be more interesting...

OK, time for my medecine.

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Green Shaman
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Cool


   Oooops, I was about to forget...
Look at that diagram and tell me what you think about it. It shows the 4 "groups" Small et all would recognize for Cannabis, as a single species of course.

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strawdog
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Originally posted by Green Shaman
Come on, what you mention here has happened naturally and artificially simultaneously or not in lots of different places, not just Afghanistan.


The reason why I am saying this is because you said...

"I believe I MUST post a pic that will illustrate my point of view better. So here's an original pure Indica, an Afghan cultivar in Mazar-e-Sharif. Supposedly a pure Indica hash plant, then. I'll let you check the type of leaf, internodal distance, and floral cluster type (from what you can guess on that pic)."

A supposedly pure Indica Hash plant which obviously has some Sativa influence. I am just pointing out that it looks like a recent Indica/Sativa hybrid, no a pure indica plant.



If two distinct populations that are also two very different types aren't in contact with each other, as is the case with many valley plants and their alpine neighboors, each type preserves its "typical" traits. But it takes nothing more than a gust of wind to change that, unless one type can really not live and prosper in the other environment. Like, the alpine type can grow in valleys but the lowland type will die up in the mountains.


What you must remember is that something like a gust of wind should have happened long ago, thus the contact would have taken place a long time ago. If the contact takes place in the now then it is a first or if they had made contact in the past then evolutionary development should be evident somewhere or the offspring may have been purged, or some of the offspring purged etc. Anyway the point to make here what happens in the now is more than likely a man made influence rather than nature.


Also, what do you call a "wild" type, and how do you tell a "wild" indigenous plant from a feral population that escaped from cultivated fields, even if it happened long ago? About when do you consider a population "wild" and not "escaped" anymore?


It you look at Vavilov's work he explains how this is done. In fact bonanists do it all the time by comparing the 'new' species/strain with what is already out there - simple as that. If it ain't out there then it is new. Vavilov found that with his wild Indica. Landrace strains show domesticated traits that are not apparent in their wild counterparts. It is done by comparison.


It is also funny that the work you mention does not refer to "wild Sativa". Does this mean the alpine "Sativas" that grow naturally in lots of places in the Himalayan where no man has cultivated them before, and where cultivated types can't survive, aren't wild plants?


Wild plants have no obvious man-made influences.
Landrace is wild, but has been influenced by man and is then left to selective breeding pressures in the wild.
Landrace or Wild when influenced by man, remaining under man's influence, is domesticated.


The truth is, there's a lot of BS in all this. I have no personal opinion on Mr Vavilov's work. I also know that there is a "Russian" "school" of scientists, all pleading in favor of a split between at least two distinct subspecies of Cannabis, vs "the rest of the world" scientists, who don't agree. Shall we exchange links regarding Mr Vavilov's studies and how they are questioned, to say the least, by other prominent botanists?


Vavilov is a legend in the world of cannabis botany. He is the one that actually went out to make observations of the cannabis plant in both dometicated and natural habitats. He followed up on the discoveries of others which where nothing more than mere samples to sent to various botany students. Vavilov studied all the various types of cannabis in Afghanistan and his own country. R. Clarke has a couple of hundred pages on the web devoted to the man.


don't want to sound rude, but Vavilov's work mentions just what I've posted here too. Let me quote him (I hope he won't mind, now): "series of races of the typical wild and typical cultivated forms of hemp show many overlapping characters", and also that all transitional forms from wild hemp to cultivated hemp can be found, and that for many forms "the morphological limits between weed hemp and true wild hemp are difficult to draw, and in regard to the characters of the fruits, the flowers and the leaves, they are indistinguishable from each other".


Okay I see where you are coming from now. Mr. Small's little 1975 paper on why cannabis should be treated as one species "American law and the species problem in Cannabis: Science and semantics" was a propaganda tool devised to prevent Americans from growing Indica who where contesting that it was not cannabis sativa. To be honest Mr. Small's paper lacks foundation because he defines 'species' and we know that 'species' actually has no defintion.


 

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Greenz
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Greenz

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Originally posted by Green Shaman

You should really abandon these concepts of "Sativa" and "Indica", and Vavilov was certainly not the best reference to mention here. Now a debate between Shultes' work and was someone like E. Small had to say about it in 1975 would certainly be more interesting...


You're right. Of course, when say "indica", I mean Cannabis sativa indica.
Same with sativa, I mean Cannabis sativa sativa.

Then there is Cannabis sativa ruderalis.
See, that's another thing we should talk about.
How long would it take for an "indica" or "sativa" population to take on "ruderalis" characteristics in a higher lattitude environment?

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The problem is Taxonomy.



Humans seem to want to lable everything.



Sometimes life is not black and white.



So, we get arguments over what constitutes a "species."


Wanna get a real mindfuck on the subject... pick up Darwins "Origins of Species." I got free beans for anyone who actually reads it AND comprehends it. LOL.


I'm not sure Darwin even thought species was a cut and dried issue.

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strawdog
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Darwin himself pretty much said that alot of it was a problem.

He had never observed reminants of past dinosaurs or ape-men which SHOULD BE HERE according to his theory (they should actually be here according to our modern also), however we have found fish that are millions of years old and have not evolved much so he was on the money there.

Also he stated that life could come from dead tissue - an observation of larva being produced from meat (flies had just laid their eggs there). He also said that certain traits could be inherited when they where not traits at all.

Mutation theory is where it is at now.


 

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Last edited by strawdog on November 10th, 2004 at 09:43 PM

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Green Shaman
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Not propaganda, Strawdog, just common sense; don't let the legal context make you miss the important conclusion, that is also accepted by contradictors:

It is fairly easy to point out differences between a few selected geographical types of cannabis, or between plants cultivated for fibers and plants cultivated for drug (in some cases...), but it's impossible to study the entire spectrum of variation of this species and define only two or three "subspecies". Again, because many characters overlap bewteen different types, you can't find reliable ID criteria. And even if you managed to create a collection of absolutely all the cultigens you could find, you couldn't separate them in only two or three main groups.

Taxonomy isn't carved into stone. New techniques appear that make scientists reconsider what seemed granted.

However, the current possibilities of science do not permit a split between several species of Cannabis, and because of the high variability of populations, it is not even possible to separate the types in only a couple of "subspecies". All you can do, at best, is trace back the history and genetic lineage of a population. This will show you how it is different from another population, but that's all. You won't find enough things in common between several populations to separate them from others at the level of a species or even a "race". Because you won't be able to point out what defines that "race", really.

This is a fact, you can't ignore it. All the field work, that is very valuable, that took place only makes this problem more evident: too much diversity.

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Uncle Bug
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Uncle Bug

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A recent genetic study by Hillig shows that there are two major gene pools of Cannabis with different geographic ranges. The indica gene pool includes both narrow leaflet "sativa" and wide leaflet "indica" drug strains. It also includes eastern Asian hemp (fiber/seed) landraces and wild or feral Himalayan populations. The sativa gene pool includes hemp landraces from Europe and central Asia, and feral European populations. It might also include "ruderalis" populations from central Asia. Both gene pools appear to have accumulated mutations over a long period of time.

Last edited by Uncle Bug on March 31st, 2005 at 03:38 PM

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Based on what? Chemotype? This looks awfully like the old "fiber vs drug" types thing... All oriental "intoxicant and semi-intoxicant" types being considered "Indica", and the non-intoxicant types being "Sativa" (Cannabis sativa var. sativa). Ruderalis types were considered "Sativa" by Vavilov and other eastern botanists. Closer to the fiber cultivars (which were Vavilov's speciality, and are still the only types preserved in the VIR/Clarke initiative -- with only two Afghan varieties, btw) than the drug types (guess why ).

What group do the African and American continent drug types belong to, then? "Indica"? LOL

Click to Print this Old Post November 12th, 2004 12:22 PM
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strawdog
Cannabis K-9

strawdog

Registered: Aug 2000
Posts: 3557


GS - Non-drug cannabis plants do not exist pre world war II. That was invented by Warmke to stop that sailors smoking rope

Ruderalis was considered Ruderalis by Janisch.

"Cannabis ruderalis Janisch."

Look below and you will see the conflict between those who actually observed these plants in the wild and the armchair scientists who received samples.

# Cannabis gigantea hort. -> Cannabis sativa L. subsp. sativa
# Cannabis indica Lam. s -> Cannabis sativa L. subsp. indica (Lam.) E. Small & Cronquist
# Cannabis ruderalis Janisch. -> Cannabis sativa L. subsp. spontanea Serebr.
# Cannabis sativa L.
# Cannabis sativa L. f. ruderalis (Janisch.) Chu -> Cannabis sativa L. subsp. spontanea Serebr.
# Cannabis sativa L. subsp. indica (Lam.) E. Small & Cronquist A
# Cannabis sativa L. subsp. ruderalis Janisch. -> Cannabis sativa L. subsp. spontanea Serebr.
# Cannabis sativa L. subsp. sativa A
# Cannabis sativa L. subsp. spontanea Serebrjakova -> Cannabis sativa L. subsp. spontanea Serebr.
# Cannabis sativa L. var. indica Lam. -> Cannabis sativa L. subsp. indica (Lam.) E. Small & Cronquist
# Cannabis sativa L. var. spontanea Vavilov -> Cannabis sativa L. subsp. spontanea Serebr.


 

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Last edited by strawdog on November 12th, 2004 at 01:07 PM

Click to Print this Old Post November 12th, 2004 01:06 PM
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