Chlorine is the most common disinfecting chemical used.  The term chlorination is often used synonymously with disinfection.  Chlorine may be used as the element as sodium hypochlorite, or as calcium hypochlorite.

         Chlorine disinfection involves a very complex series of events and is influenced by the kind and extent of reactions with chlorine-reactive materials (including nitrogen), temperature, pH, the viability of test organisms, and numerous other factors.  Such factors greatly complicate attempts to determine the precise mode of action of chlorine on bacteria and other microorganisms.  Over the years, several theories have been advanced.  One early theory held that chlorine reacts directly with water to produce nascent oxygen; another held that the action of chlorine is due to complete oxidative destruction of organisms.  These theories were nullified because small concentrations of hypochlorous acid were observed to destroy bacteria whereas other oxidants (such as hydrogen peroxide or potassium permanganate) failed to do likewise.  A later theory suggested that chlorine reacts with protein and amino acids of cells to alter and ultimately destroy cell protoplasm such as variations in resistance of bacteria. spores, cysts, and viruses, and the appeareance of mutants.


       
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