I have developed many useful computer programs for biomedical applications including DNA microarray analysis (ArrayVigil, ArraySolver, ArrayShine), quantitation of experimental gastric lesions (Article), drug dosage conversion (CalcDose), survival curves (SCEW) and computations of Fisher�s exact probability (CalcFisher), and normal tissue complication probability (CalcNTCP).
The log-based formula developed by me has been used by the scientists of Rollins School of Public Health, Emory University, USA, to develop a very comprehensive epidemiological software (2 x 2 Table, Version 4.12.14).
Our computer-based methodology for the evaluation of gastric lesions has been used by other scientists for their research (patent-pending) on novel pharmacotherapies for the treatment of gastric ulcers.
Scientists from different countries including USA, UK, France, Germany, Czech Republic, Russia, China, Brazil, India and Saudi Arabia have shown their interest in these software tools for their research or teaching activities. These application programs are freely available to scientists and one of the software (with Russian help topics) is also available online at the website of Tomsk University, Russia http://www.biometrica.tomsk.ru/programm_stat.htm
Our clinical and basic research projects involve multidisciplinary areas like clinical biochemistry, molecular biology, pharmacology and toxicology. Recently we have evaluated the utility of combined values of serum fructosamine and fasting/random blood glucose for identification of high risk diabetic patients. We have also suggested the efficacy of HbA1c in predicting dyslipidemia besides its primary role in monitoring long-term glycemic control.
The findings of our basic research have shown the protective effects of anti-inflammatory and antioxidant drugs in experimental gastric lesions. We have noticed that drugs with the ability to down-regulate inflammatory cascade can have beneficial effects in septic shock.
Using a mouse model of Parkinson�s disease, we concluded that an early-stage proinflammatory cascade may lead to subsequent neurodegeneration. An important role of phospholipase A2 (PLA2) in experimental parkinsonism was also suggested by showing significant reversal of MPTP and 6-OHDA induced dopaminergic toxicity by PLA2 inhibitor quinacrine.
In a recent investigation, we noticed that inhibition of cholinergic neurotransmission by nicotine may have beneficial effects in experimental Huntington�s disease. We also observed protective effects of dopamine antagonist and antioxidants in experimental movement disorders.
Our studes in rodents have shown that advancing age and gentamicin enhance the behavioral syndrome caused by a prototype nitrile compound. We also demonstrated protective effects of antioxidants in cyclosporine-induced nephrotoxicity and the adverse effects of aluminum and caffeine in experimental neurotrauma.
Besides above pharmacological research I have also developed methodologies for genotyping, gene expression analysis, enzyme assays, bioamiens analysis and ATP estimation.